Abstract
Mouse erythroleukaemia cells (also called Friend cells) can be isolated from the spleen of certain strains of mice that have been infected with the Friend virus complex1. The cells resemble pro-erythroblasts and, when exposed to dimethyl sulphoxide (DMSO) or a variety of other chemicals, can be induced to undergo a programme of differentiation which closely resembles the final stages of normal erythropoiesis2. This includes the cessation of proliferation and large increases in the production of messenger RNA for both α- and β-globin. In addition, DMSO induces a rapid (<2h) decrease in c-myc mRNA levels3,4. The c-myc oncogene is expressed in the majority of proliferating normal cells5 and altered expression of the gene has been implicated in the genesis of a wide variety of tumours6–9. To study the influence of oncogene activation on differentiation, we have transfected viral-promoter-driven c-myc genes into mouse erythroleukaemia cells. Constitutive c-myc expression was found to block DMSO-induced differentiation.
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Coppola, J., Cole, M. Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment. Nature 320, 760–763 (1986). https://doi.org/10.1038/320760a0
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DOI: https://doi.org/10.1038/320760a0
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