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Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes

Abstract

Transfection of embryo fibroblasts by a human ras oncogene does not convert them into tumour cells unless the fibroblasts are established and immortalized before transfection. The embryo fibroblasts become tumorigenic if a second oncogene such as a viral or cellular myc gene or the gene for the polyoma large-T antigen is introduced together with the ras gene.

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Land, H., Parada, L. & Weinberg, R. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature 304, 596–602 (1983). https://doi.org/10.1038/304596a0

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