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Antioxidant and antidiabetic properties of bioactive peptides from soursop (Annona muricata) leaf biomass

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Abstract

This study investigated the antioxidant and antidiabetic properties of soursop (Annona muricata) leaf biomass hydrolysates (SLH) extracted from the leaf proteins. The results revealed that flavourzyme-generated SLH showed the highest peptide content when compared to alcalase- and trypsin-generated SLH. Application of response surface methodology (RSM) remarkably increased peptide content under the optimized conditions at an E/S ratio of 4% (w/w), hydrolysis time of 4 h, a temperature of 50 °C, and pH of 7. SLH demonstrated stronger antioxidant (DPPH (22.92%) and ABTS (90.55%)) and antidiabetic (α-glucosidase inhibition (66.44%) and α-amylase inhibition (58.50%)) activities. A total of 14 peptides were identified as bioactive peptides, among which two peptides (FLAISPKAMK and WDSKWTHI) possessed the highest binding probability toward α-glucosidase and α-amylase. These peptides contain hydrophobic amino acids, which are important in improving the antioxidant activities of peptides. In conclusion, SLH could be useful for diabetes prevention.

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Funding

This material is based upon work supported by the Malaysia Toray Science Foundation (grant number: R.J130000.7351.4B629).

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Author notes

  1. The work was equally contributed by Chia Hau Lee, Norfadilah Hamdan, and Luo Ing Ling.

Authors and Affiliations

  1. Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Chia Hau Lee, Norfadilah Hamdan, Luo Ing Ling & Ting Hun Lee

  2. Dpto. Matemática Aplicada, Ciencia e Ingeniería de Materiales Y Tecnología Electrónica, Universidad Rey Juan Carlos, C/ Tulipán S/N, 28933, Móstoles, Madrid, Spain

    Syie Luing Wong

  3. UTM Centre for Low Carbon Transport (LoCARtic), Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Bemgba Bevan Nyakuma

  4. Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, 21030, Terengganu, Malaysia

    Shing Ching Khoo

  5. Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Bahru, Johor, Malaysia

    Heeravathy Ramachandran & Haryati Jamaluddin

  6. Faculty of Mechanical Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Keng Yinn Wong

  7. Innovation Centre in Agritechnology (ICA), Universiti Teknologi Malaysia, 84600, Muar, Johor, Malaysia

    Ting Hun Lee

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  1. Chia Hau Lee
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Lee, C.H., Hamdan, N., Ling, L.I. et al. Antioxidant and antidiabetic properties of bioactive peptides from soursop (Annona muricata) leaf biomass. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-03993-5

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