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T11TS inhibits glioma angiogenesis by modulation of MMPs, TIMPs, with related integrin αv and TGF-β1 expressions

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Tumor Biology

Abstract

During glioma development, angiogenesis plays a crucial role in growth and vascularization of primary brain tumors. T11 target structure (T11TS), a bioactive molecule, has been documented as an anti-neoplastic agent in glioma-induced rats and also in human glioma in vitro. This novel molecule induces apoptosis of tumor cells by way of immune potentiation and impairs the glioma cell cycle, but its role in glioma angiogenesis has not been worked out in detail. Matrix metalloproteinases (MMPs) are enzymes promoting tumor angiogenesis by enzymatically remodeling the extracellular matrix and altering surface protein expression such as integrin αv and the matrix-bound proteins like TGF-β1. The present study was formulated to assess the efficacy of T11TS in the modulations of MMP-2 and −9 and their endogenous inhibitors (TIMP-1 and TIMP-2) as well as modulations of integrin αv and TGF-β1 in glioma-induced rats and also on the phenotypic markers of endothelial cells (CD31 and CD34). The parameters used were zymography, western blot, and flow cytometric analyses. It was observed that T11TS administration significantly downregulates the expression of matrix metalloproteinase-2 and −9 along with its ligand integrin αv and upregulates TIMP-1 and TIMP-2. In situ immunofluorescence and FACS results revealed that T11TS administration decreased the expression of the phenotypic markers (CD31/PECAM1, CD34), inhibiting the cell grip and also downregulating TGF-β1 expression (ELISA) from microglia cells in the glioma microenvironment. These results suggest that T11TS suppresses the expression of positive angiogenic growth factors and potentiates the expression of negative regulators in glioma-associated endothelial cells (ECs), resulting in an anti-angiogenic effect on glioma-induced angiogenesis.

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Acknowledgments

This study was supported a research grant from DST Govt. of India [F. No.SR/SO/HS/-16/2007, 2008]. The authors are also grateful to Dr. Sirshendu Chatterjee for significant technical support.

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Authors and Affiliations

  1. Department of Laboratory Medicine, School of Tropical Medicine, 108, C.R. Avenue, Kolkata, 700073, West Bengal, India

    Manoj Kumar Singh, Debanjan Bhattacharya, Suhnrita Chaudhuri, Pankaj Kumar, Pranjal Santra & Swapna Chaudhuri

  2. Department of Zoology, Vidyasagar University, West Midnapore, West Bengal, India

    Sagar Acharya

  3. Department of Biochemistry and Medical Biotechnology, School of Tropical Medicine, 108, C.R. Avenue, Kolkata, 700073, West Bengal, India

    Anjan Kumar Basu

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  1. Manoj Kumar Singh
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Correspondence to Swapna Chaudhuri.

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Singh, M.K., Bhattacharya, D., Chaudhuri, S. et al. T11TS inhibits glioma angiogenesis by modulation of MMPs, TIMPs, with related integrin αv and TGF-β1 expressions. Tumor Biol. 35, 2231–2246 (2014). https://doi.org/10.1007/s13277-013-1296-8

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