Abstract
Purpose
This work aimed the food waste (FW) valorization by the production of fungal enzymes in solid-state fermentation (SSF) and a hydrolysate with the potential to obtain products with high added value.
Methods
An original bioprocess based on Rhizopus oligosporus cultured by SSF using medium consisting of FW, sugarcane bagasse (SCB), wheat bran (WB), and corn steep liquor (CSL) utilizing a cell-substrate recycling system was implemented.
Results
The highest outcomes were achieved using FW as the main substrate and the strategy of one cell recycling round. The best condition for amylase production (260.9 U/g) was the blend FW 50%, SCB 10%, WB 40% supplemented with a salt solution. For the highest protease level (665.5 U/g) the same mixture was supplemented by 20% CSL. Hydrolysis of FW with the enzymatic extract from the best specified blend produced 47.10 g/L of reducing sugars.
Conclusion
This bioprocess showed to be cost-effective, technically not demanding, and could be scaled up successfully for commercial goals.
Graphical Abstract
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Data Availability
All data generated or analysed during this study are included in this published article.
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Acknowledgements
The authors are thankful to the Brazilian Council for Research and Development [CNPq, Brazil, Grant Number 141472/2015-4] for Ph.D. Studentship and São Paulo Research Foundation [FAPESP, SP, Brazil, Grant Numbers 2014/241881, 2017/029097 and 2018/045076] for the funding. The first author acknowledges her parents, Durval and Neide, and brother, Gustavo for the inspiration to write this paper.
Funding
The study was funded by Brazilian Council for Research and Development [CNPq, Brazil, Grant Number 141472/2015–4] and São Paulo Research Foundation [FAPESP, SP, Brazil, Grant Numbers 2014/241881, 2017/029097 and 2018/045076].
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PON and BE conceived and designed the research. BE, BCG, and MMA conducted experiments as well as organized and analyzed the data. BE and BCG wrote the manuscript. DAP contributed to the cost-effectiveness analysis and reviewed the manuscript. EGFN contributed with data statistical processing, reviewed and edited the paper. All authors read and approved the manuscript.
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Escaramboni, B., Garnica, B.C., Abe, M.M. et al. Food Waste as a Feedstock for Fungal Biosynthesis of Amylases and Proteases. Waste Biomass Valor 13, 213–226 (2022). https://doi.org/10.1007/s12649-021-01511-0
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DOI: https://doi.org/10.1007/s12649-021-01511-0