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Fructose-rich diet-induced abdominal adipose tissue endocrine dysfunction in normal male rats

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Abstract

We have currently studied the changes induced by administration of a fructose-rich diet (FRD) to normal rats in the mass and the endocrine function of abdominal (omental) adipose tissue (AAT). Rats were fed ad libitum a standard commercial chow and tap water, either alone (control diet, CD) or containing fructose (10%, w/vol) (FRD). Three weeks after treatment, circulating metabolic markers and leptin release from adipocytes of AAT were measured. Plasma free fatty acids (FFAs), leptin, adiponectin, and plasminogen activator inhibitor-1 (PAI-1) levels were significantly higher in FRD than in CD rats. AAT mass was greater in FRD than in CD rats and their adipocytes were larger, they secreted more leptin and showed impaired insulin sensitivity. While leptin mRNA expression increased in AAT from FRD rats, gene expression of insulin receptor substrate, IRS1 and IRS2 was significantly reduced. Our study demonstrates that administration of a FRD significantly affects insulin sensitivity and several AAT endocrine/metabolic functions. These alterations could be part of a network of interacting abnormalities triggered by FRD-induced oxidative stress at the AAT level. In view of the impaired glucose tolerance observed in FRD rats, these alterations could play a key role in both the development of metabolic syndrome (MS) and β-cell failure.

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Acknowledgments

This study was supported by grants from FONCyT (PICT-2007-01051 to ES), Fondation de Recherche en Endocrinologie (2006/2008; to ES), and FNSR (3200BO-105657/1; to RCG). ES, JJG, and AG are members of the Research Career of CONICET; ORR is a member of the National University Incentives Program; AA, AR, and VM are CONICET fellows.

The authors gratefully thank D. Castrogiovanni for his excellent technical assistance, and A. Di Maggio and S. Rogers for manuscript edition and correction, respectively.

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Authors and Affiliations

  1. Neuroendocrine Unit, IMBICE (CONICET-CICPBA), PO Box 403, 1900, La Plata, Argentina

    Ana Alzamendi, Andrés Giovambattista & Eduardo Spinedi

  2. CENEXA (UNLP-CONICET, PAHO/WHO Collaborating Center), La Plata, Argentina

    Agustina Raschia, Viviana Madrid, Oscar Rebolledo & Juan J. Gagliardino

  3. Division of Endocrinology, Diabetology and Metabolism, University Hospital (CHUV), CH-1011, Lausanne, Switzerland

    Rolf C. Gaillard

Authors
  1. Ana Alzamendi
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  2. Andrés Giovambattista
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  3. Agustina Raschia
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  4. Viviana Madrid
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  5. Rolf C. Gaillard
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  6. Oscar Rebolledo
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  7. Juan J. Gagliardino
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  8. Eduardo Spinedi
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Correspondence to Eduardo Spinedi.

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Alzamendi, A., Giovambattista, A., Raschia, A. et al. Fructose-rich diet-induced abdominal adipose tissue endocrine dysfunction in normal male rats. Endocr 35, 227–232 (2009). https://doi.org/10.1007/s12020-008-9143-1

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  • DOI: https://doi.org/10.1007/s12020-008-9143-1

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