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Effects of 28-homobrassinolide on growth, lipid peroxidation and antioxidative enzyme activities in seedlings of Zea mays L. under salinity stress

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Abstract

The effects of 28-homobrassinolide (28-homoBL) on seedling growth, lipid peroxidation and antioxidative enzyme activities in the seedlings of Zea mays L. (var. Partap-1) under salt (NaCl) stress were studied. The surface-sterilized seeds were germinated in petriplates containing different concentrations of NaCl (25, 50, 75 and 100 mM) only, 28-homoBL (10−7, 10−9 and 10−11 M) only and NaCl supplemented with 28-homoBL for 7 days. The activities of superoxide dismutase (SOD, EC 1.15.1.1), guaiacol peroxidase (POD, EC 1.11.1.7), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APOX, EC 1.11.1.11) and glutathione reductase (EC 1.6.4.2) were analysed in 7 day-old seedlings. It was observed that 28-homoBL treatments reduced the toxicity of salt on seedling growth considerably. Lipid peroxidation level was significantly increased under saline stress, but lowered with 28-homoBL applications revealing less oxidative damage. Further 28-homoBL treatments to the seedlings showed an enhancement in activities of SOD, POD, CAT and APOX. The activities of all antioxidative enzymes were further increased in seedlings treated with solution containing 28-homoBL and salt together as compared to seedlings treated with different concentration of salt solution only.

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Abbreviations

28-homoBL:

28-Homobrassinolide

APOX:

Ascorbate peroxidase

BRs:

Brassinosteroids

CAT:

Catalase

24-epiBL:

24-Epibrassinolide

GR:

Glutathione reductase

MDA:

Malondialdehyde

POD:

Guaiacol peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

References

  • Aebi H (1983) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 2. Verlag Chemie, Weinheim, pp 673–684

    Google Scholar 

  • Ali B, Hayat S, Ahmad A (2005) Response of germinating seeds of Cicer arietinum to 28-homobrassinolide and/or potassium. Gen Appl Plant Physiol 31:55–63

    CAS  Google Scholar 

  • Anuradha S, Rao SSR (2001) Effect of brassinosteroids on salinity stress induced inhibition of germination and seedling growth of rice (Oryza sativa L.). Plant Growth Regul 33:151–153

    Article  CAS  Google Scholar 

  • Anuradha S, Rao SSR (2003) Application of brassinosteroids to rice seeds (Oryza sativa L.) reduced the impact of salt stress on growth, prevented photosynthetic pigment loss and increased nitrate reductase activity. Plant Growth Regul 40:29–32

    Article  CAS  Google Scholar 

  • Arora A, Sairam RK, Srivastava GC (2002) Oxidative stress and antioxidative system in plants. Curr Sci 82:1227–1238

    CAS  Google Scholar 

  • Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CJ, Artzen CJ (eds) Photoinhibition: topics in photosynthesis. Elsevier, Amsterdam, pp 227–287

    Google Scholar 

  • Bajguz A, Tretyn A (2003) The chemical characteristic and distribution of brassinosteroids in plants. Phytochemistry 62:1027–1046

    Article  PubMed  CAS  Google Scholar 

  • Bhardwaj R, Arora HK, Nagar PK, Thukral AK (2006) Brassinosteroids—a novel group of plant hormones. In: Trivedi PC (ed) Plant molecular physiology—current scenario and future projections. Aavishkar Publisher, Jaipur, pp 58–84

    Google Scholar 

  • Bhardwaj R, Arora N, Sharma P, Arora HK (2007) Effects of 28-homobrassinolide on seedling growth, lipid peroxidation and antioxidative enzyme activities under nickel stress in seedlings of Zea mays L. Asian J Plant Sci 6(5):765–772

    Article  CAS  Google Scholar 

  • Cao S, Xu Q, Cao Y, Quian K, An K, Zhu Y, Bineng H, Zhao H, Kuai B (2005) Loss of function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. Physiol Plant 123:57–66

    Article  CAS  Google Scholar 

  • Carlberg I, Mannervik B (1975) Purification of the flavoenzyme glutathione reductase from rat liver. J Biol Chem 250:5475–5480

    PubMed  CAS  Google Scholar 

  • Cerana R, Bonetti A, Narre MT, Romani G, Lado P, Marre E (1983) Effects of brassinosteroid on growth and electrogenic proton extrusion in Azuki bean epicotyls. Physiol Plant 59:23–27

    Article  CAS  Google Scholar 

  • Cerana R, Lado P, Anastasia M, Ciuffreda P, Allevi P (1984) Regulating effects of brassinosteroids and of sterols on growth and H+ secretion in maize roots. J Plant Physiol 114:221–225

    CAS  Google Scholar 

  • Cheeseman JM (1988) Mechanisms of salinity tolerance in plants. Plant Physiol 87:547–550

    PubMed  CAS  Google Scholar 

  • Clouse SD, Sasse JM (1998) Brassinosteroids: essential regulators of plant growth and development. Annu Rev Plant Physiol Plant Mol Biol 49:427–451

    Article  PubMed  CAS  Google Scholar 

  • Dhaubhadel S, Chaudhary S, Dobinson KF, Krishna P (1999) Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings. Plant Mol Biol 40:333–342

    Article  PubMed  CAS  Google Scholar 

  • Dhaubhadel S, Browning KS, Gallie DR, Krishna P (2002) Brassinosteroid functions to protect the translational machinery and heat shock protein synthesis following thermal stress. Plant J 29(6):681–691

    Article  PubMed  CAS  Google Scholar 

  • Haubrick LL, Assmann SM (2006) Brassinosteroids and plant function: some clues, more puzzles. Plant Cell Environ 29:446–457

    Article  PubMed  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  PubMed  CAS  Google Scholar 

  • Khripach V, Zhabinskii V, Groot AD (2000) Twenty years of brassinosteroids: steroidal plant hormones warrant better crops for XXI century. Ann Bot 86:441–447

    Article  CAS  Google Scholar 

  • Kono Y (1978) Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Biophys 186:189–195

    Article  PubMed  CAS  Google Scholar 

  • Krishna P (2003) Brassinosteroids—mediated stress responses. J Plant Growth Regul 22:289–297

    Article  PubMed  CAS  Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  • Mandava NB (1988) Plant growth promoting brassinosteroids. Annu Rev Plant Physiol Plant Mol Biol 39:25–52

    Article  Google Scholar 

  • McCord JM (2000) The evolution of free radicals and oxidative stress. Am J Med 108:652–659

    Article  PubMed  CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5):867–880

    CAS  Google Scholar 

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279

    Article  PubMed  CAS  Google Scholar 

  • Nunez M, Mazzafera P, Mazorra LM, Siqueira WJ, Zullo MAT (2003) Influence of brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl. Biol Plant 47:67–70

    Article  CAS  Google Scholar 

  • Özdemir F, Bor M, Demiral T, Turkan I (2004) Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress. Plant Growth Regul 42:203–211

    Article  Google Scholar 

  • Putter J (1974) Peroxidase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 2. Verlag Chemie, Weinheim, pp 685–690

    Google Scholar 

  • Rao SSR, Vardhini BV, Sujatha E, Anuradha S (2002) Brassinosteroids—a new class of phytohormones. Curr Sci 82:1239–1245

    Google Scholar 

  • Sairam RK, Tyagi A (2004) Physiology and molecular biology of salinity stress tolerance in plants. Curr Sci 86(3):407–421

    CAS  Google Scholar 

  • Salin ML (1988) Toxic oxygen species and protective systems of chloroplast. Physiol Plant 72:681–728

    Article  CAS  Google Scholar 

  • Sharma P, Bhardwaj R (2007) Effects of 24-epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress. Acta Physiol Plant 29:259–263

    Article  CAS  Google Scholar 

  • Upreti KK, Murti GSR (2004) Effects of brassinosteroids on growth, nodulation, phytochrome content and nitrogenase activity in French bean under water stress. Biol Plant 48(3):407–411

    Article  CAS  Google Scholar 

  • Wachsman MB, Lopez EM, Ramirez JA, Galagovsky LR, Coto CE (2000) Antiviral effect of brassinosteroids against herpes virus and arenaviruses. Antivir Chem Chemother 11:71–77

    PubMed  CAS  Google Scholar 

  • Wachsman MB, Ramirez JA, Galagovsky LR, Coto CE (2002) Antiviral activity of brassinosteroids derivatives against measles virus in cell cultures. Antivir Chem Chemother 13:61–66

    PubMed  CAS  Google Scholar 

  • Wachsman MB, Ramirez JA, Talarico LB, Galagovsky LR, Coto CE (2004) Antiviral activity of natural and synthetic brassinosteroids. Curr Med Chem Anti-Infect Agents 3:163–179

    Article  CAS  Google Scholar 

  • Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218:1–14

    Article  PubMed  CAS  Google Scholar 

  • Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14:165–183

    Article  CAS  Google Scholar 

  • Zhang CF, Hu J, Lou J, Zhang Y, Hu WM (2007a) Sand priming in relation to physiological changes in seed germination and seedling growth of waxy maize under high salt stress. Seed Sci Technol 35(3):733–738

    Google Scholar 

  • Zhang S, Hu J, Zhang Y, Xie XJ, Knapp A (2007b) Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress. Aust J Agric Res 58(8):811–815

    Article  CAS  Google Scholar 

  • Zhu JK (2001) Over expression of delta-pyrroline-5-carboxylate synthetase gene and analysis of tolerance to water and salt stress in transgenic rice. Trends Plant Sci 6:66–72

    Article  PubMed  CAS  Google Scholar 

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Acknowledgment

Financial assistance from University Grants Commission, New Delhi, India, is duly acknowledged.

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Authors and Affiliations

  1. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, 143005, Punjab, India

    Nitika Arora, Renu Bhardwaj, Priyanka Sharma & Hardesh Kumar Arora

Authors
  1. Nitika Arora
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  2. Renu Bhardwaj
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  3. Priyanka Sharma
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  4. Hardesh Kumar Arora
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Corresponding author

Correspondence to Renu Bhardwaj.

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Communicated by S. Lewak.

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Arora, N., Bhardwaj, R., Sharma, P. et al. Effects of 28-homobrassinolide on growth, lipid peroxidation and antioxidative enzyme activities in seedlings of Zea mays L. under salinity stress. Acta Physiol Plant 30, 833–839 (2008). https://doi.org/10.1007/s11738-008-0188-9

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  • DOI: https://doi.org/10.1007/s11738-008-0188-9

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