biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:407-411, 2004 | DOI: 10.1023/B:BIOP.0000041094.13342.1b

Effects of Brassmosteroids on Growth, Nodulation, Phytohormone Content and Nitrogenase Activity in French Bean Under Water Stress

K.K. Upreti1, G.S.R. Murti1
1 Plant Hormones Laboratory, Indian Institute of Horticultural Research, Bangalore, India, e-mail

Effect of pre-treatments of 1 and 5 μM epibrassinolide or homobrassinolide prior to water stress induction on changes in root nodulation and contents of endogenous abscisic acid (ABA) and cytokinin trans-zeatin riboside (ZR), and nitrogenase activity was investigated in the nodulated roots of Phaseolus vulgaris L. cv. Arka Suvidha. Brassinosteroids in the unstressed plants increased root nodulation, ZR content and nitrogenase activity, and also ameliorated their stress-induced decline in the nodulated roots. The ABA contents in the nodules of control or stressed plants were not altered by brassinosteroids treatment. There was an increase in pod yield by brassinosteroids treatment (5 μM) in the irrigated control as well as stressed plants without influencing pod number or pod length. Among the brassinosteroids, epibrassinolide was relatively more effective.

Keywords: abscisic acid; cytokinins; drought; Phaseolus vulgaris; yield
Subjects: abscisic acid (ABA); brassinosteroid, nodulation, nitrogenase; cytokinins; French bean, brassinosteroids; growth, brassinosteroids; nitrogen fixation, brassinosteroid; nitrogenase, nodulation; nodulation, nodules; Phaseolus vulgaris; water stress, brassinosteroids, nodulation

Published: September 1, 2004  Show citation

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Upreti, K.K., & Murti, G.S.R. (2004). Effects of Brassmosteroids on Growth, Nodulation, Phytohormone Content and Nitrogenase Activity in French Bean Under Water Stress. Biologia plantarum, 48(3), 407-411. doi: 10.1023/B:BIOP.0000041094.13342.1b
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    References

    1. Arteca, R.N.: Jasmonates, salicylic acid, brassinosteroids.-In: Davies, P.J. (ed.): Plant Hormones: Physiology, Biochemistry and Molecular biology. Pp. 206-213. Kluwer Acad. Publ., Dordrecht 1995.
    2. Bano, A., Watts, S.H., Hillman, J.R., Wheele, C.T.: Abscisic acid and nitrogen fixation in Faba vulgaris (Vicia faba) and Alnus glutinosa.-J. Sci. Food Agr. 34: 941-942, 1983.
    3. Barthe, G.A., Stewart I.: Enzyme immunoassay (EIA) of endogenous cytokinins in citrus.-J. Agr. Food Chem. 33: 293-297, 1985. Go to original source...
    4. Bordeleau, L.M., Prevost, D.: Nodulation and nitrogen fixation in extreme environments.-Plant Soil 161: 115-125, 1994. Go to original source...
    5. Cho, M.J., Harper, J.E.: Effect of abscisic acid application on root isoflavonoid concentration and nodulation of wild-type and nodulation-mutant soybean plants.-Plant Soil 153: 145-149, 1993. Go to original source...
    6. Clouse, S.D., Sasse, J.M.: Brassinosteroids: Essential regulators of plant growth and development.-Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 427-451, 1998. Go to original source...
    7. Davies, W.J., Zhang, J.: Root signals and the regulation of growth and development of plants in drying soil.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 55-76, 1991. Go to original source...
    8. Fang, Y., Hirsch, A.M.: Studying early nodulin gene ENOD40 expression and induction by nodulation factor and cytokinin in transgenic alfalfa.-Plant Physiol. 116: 53-68, 1998. Go to original source...
    9. Fedorova, E.E., Zhiznevskaya, G.Y., Al'zhapparova, Z.K., Izmailov, S.F.: [Phytohormones in nitrogen-fixing nodules of leguminous plants.]-Fiziol. Biokhim. Rast. 23: 426-438, 1991. [In Russ.]
    10. Fli, H., Vessey, J.K.: Involvement of cytokinin in the stimulation of nodulation by low concentrations of ammonium in Pisum sativum.-Physiol. Plant. 118: 447-455, 2003. Go to original source...
    11. Garg, N., Garg, O.P., Dua, I.S.: Effect of gibberellic acid and kinetin on nodulation and nitrogen fixation in chick pea (Cicer arietinum L.).-Indian J. Plant Physiol. 35: 401-404, 1992.
    12. Gonzalez, E.M., Aparicio-Tejo, P.M., Gordon, A.J., Minchin, F.R., Royuela, M., Arrese-Igor, C.: Water-deficit effects on carbon and nitrogen metabolism of pea nodules.-J. exp. Biol. 49: 1705-1714, 1998. Go to original source...
    13. Hardy, R.W., Holsten, R.D., Jackson, E.K., Burns, R.C.: The acetylene-ethylene assay for N2 fixation: Laboratory and field evaluation. - Plant Physiol. 43: 1185-1207, 1968. Go to original source...
    14. Itai, C.: Role of phytohormones in plant responses to stresses.-In: Lerner, H.R. (ed.): Plant Response to Environmental Stress: From Phytohormones to Genome Reorganization. Pp. 287-301, Marcel Dekker, New York 1999. Go to original source...
    15. Khripach, V., Zhabinskii, V., De Groot, A.: Twenty years of brassinosteroids: Steroidal plant hormones warrant better crops for the XXI century.-Ann. Bot. 86: 441-447, 2000. Go to original source...
    16. Lorteau, M.A., Ferguson, B.J., Guinel, F.C.: Effects of cytokinin on ethylene production and nodulation in pea (Pisum sativum) cv. Sparkle. - Physiol. Plant. 112: 421-428, 2001. Go to original source...
    17. Philips, D.A.: Abscisic acid inhibition of root nodule initiation in Pisum sativum.-Planta 100: 181-190, 1971. Go to original source...
    18. Ramos, M.L.G., Gordon, A.J., Minchin, F.R., Sprent, J.I., Parsons, R.: Effect of water stress on nodule physiology and biochemistry of a drought tolerant cultivar of common bean (Phaseolus vulgaris L.).-Ann. Bot. 83: 57-63, 1999. Go to original source...
    19. Sasse, J.M.: Recent progress in brassinosteroid research.-Physiol. Plant. 100: 696-701, 1997. Go to original source...
    20. Serraj, R., Sinclair, T.R., Allen, L.H.: Soybean nodulation and N2 fixation response to drought under carbon dioxide enrichment.-Plant Cell Environ. 21: 491-500, 1998. Go to original source...
    21. Serraj, R., Sinclair, T.R., Purcell, L.C.: Symbiotic N2 fixation response to drought.-J. exp. Bot. 50: 143-155, 1999. Go to original source...
    22. Singh, T., Kumar, V., Singh, T.: Nodulation and plant growth as influenced by growth regulators in some legumes-Acta bot. indica 17: 177-181, 1989.
    23. Taller, B.J.: Cytokinins and legume nodulation.-In: Gresshoff, P.M (ed.): Plant Biotechnology and Development. Pp. 55-60, CRC Press, Boca Raton 1992.
    24. Upreti, K.K., Murti, G.S.R.: Water stress-induced changes in root nodulation and cytokinin levels in French bean.-J. Plant Biol. 26: 187-190, 1999. Go to original source...
    25. Upreti, K.K., Murti, G.S.R., Bhatt, R.M.: Response of French bean cultivars to water deficits: changes in endogenous hormones, proline and chlorophyll.-Biol. Plant. 40: 381-388, 1998. Go to original source...
    26. Vardhini, B.V., Rao, S.S.R.: Effect of brassinosteroids on nodulation and nitrogenase activity in groundnut (Arachis hypogaea L.).-Plant Growth Regul. 28: 165-167, 1999. Go to original source...
    27. Venkateswarlu, B., Saharan, N., Maheswari, M.: Nodulation and N2 (C2H2) fixation in cowpea and groundnut during water stress and recovery.-Field Crops Res. 25: 223-232, 1990. Go to original source...
    28. Weiler, E.W.: An enzyme-immunoassay for cis-(+)-abscisic acid.-Physiol. Plant. 54: 510-514, 1982. Go to original source...
    29. Williams, P.M., Sicardi-de-Mallorca, M.: Effect of osmotically induced leaf moisture stress on nodulation and nitrogenase activity of Glycine max.-Plant Soil 80: 267-283, 1984. Go to original source...

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