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Changes of phenotype and function of human CD4+ CD25− T cells induced by transfection of Foxp3

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Abstract

The aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and function of naive CD4+ T cells. The pMSCV-Foxp3 retroviral vector encoding Foxp3 gene was transduced into the PT67 packaging cell line. Virus-containing supernatant was applied to differentiate CD4+CD25− T cells. The resulting cells were sorted with flow cytometry. The expressions of CD25, CD127, CTLA-4 and the proliferation of transfected T cells were examined. The effect of transfected CD4+ T cells on the proliferation and cytokine production of CD4+CD25− T cells was examined. Foxp3-gene transfected CD4+ T cells could express Foxp3 and transfection of Foxp3 gene up-regulated the expressions of CD25 and CTLA-4, but down-regulated CD127 expression. After transfection, the proliferation of CD4+ T cells was eliminated. Transfected T cells inhibited the proliferation of CD4+CD25− T cells. CD4+CD25− T cells acquired a regulatory phenotype and function after it was transduced with the Foxp3 gene. This suggested a key role of Foxp3 in the generation of CD4+CD25+ regulatory T cells.

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Authors and Affiliations

  1. Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

    Kui Wu, Yutian Bi, Yaoli Wang & Changzheng Wang

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  1. Kui Wu
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  2. Yutian Bi
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  3. Yaoli Wang
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  4. Changzheng Wang
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Correspondence to Changzheng Wang.

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Translated from Acta Academiae Medicinae Militaris Tertiae, 2008, 30(3): 186–188 [译自: 第三军医大学学报]

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Wu, K., Bi, Y., Wang, Y. et al. Changes of phenotype and function of human CD4+ CD25− T cells induced by transfection of Foxp3. Front. Med. China 2, 366–369 (2008). https://doi.org/10.1007/s11684-008-0070-6

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