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Anti-skin-aging effects of tissue-cultured mountain-grown ginseng and quantitative HPLC/ELSD analysis of major ginsenosides

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Abstract

Mountain-grown ginseng has free radical scavenging activity and suppresses inflammation. We evaluated the anti-skin-aging effects of tissue-cultured mountain-grown ginseng (TG) and its major ginsenosides. The effect of three extracts of TG and ginsenosides Rg1 (1), Rf (2), Rb1 (3), Re (4), and Rd (5) on the secretion of matrix metalloproteinase-1 (MMP-1) and collagen type I alpha 1 (COLIA1) was compared with that of tumor necrosis factor-alpha (TNF-α) stimulation of human dermal fibroblasts (HDFs), as determined via enzyme-linked immunosorbent assay. An analytical high-performance liquid chromatographic method with evaporative light-scattering detection (HPLC/ELSD) was developed for the simultaneous determination of the major ginsenosides in TG obtained via supercritical fluid CO2 or ethanol extraction. TG residues obtained via supercritical fluid CO2 extraction (TG1) and TG not subject to extraction (TG3) suppressed MMP-1 secretion in TNF-α-stimulated HDFs. Major ginsenoside content was higher in the TG1 than in residues extracted with ethanol (TG2) and TG3; ginsenoside Rg1 (1) content was the highest among all TG residues. Among them, ginsenosides Rg1 (1) and Re (4) suppressed MMP-1 in TNF-α-stimulated HDFs, whereas ginsenosides Rb1 (3) and Rd (5) increased COLIA1. In conclusion, TG and its active ginsenosides may have anti-skin-aging effects. Ginsenoside Rg1 (1) may also be beneficial in ameliorating skin damage. HPLC/ELSD can identify major ginsenosides and supercritical fluid CO2 extraction can be applied during health supplement or drug development.

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Acknowledgements

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (iPET) through the Technology Commercialization Support Program and Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Korea (grant numbers: 817022-3 and 112044-3).

Funding

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (iPET) through the Technology Commercialization Support Program and Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Korea (grant numbers:817022-3 and 112044-3).

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  1. Juree Kim and Hung Manh Phung are contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Science and Technology, Chung-Ang University, Anseong, 17546, Korea

    Juree Kim & Sanghyun Lee

  2. Department of Preventive Medicine, Gachon University, Seongnam, 13120, Korea

    Hung Manh Phung & Ki Sung Kang

  3. Department of Life Science, Gachon University, Seongnam, 13120, Korea

    Sullim Lee

  4. Ximin Co. Ltd, Daegu, 42809, Korea

    Keun Tae Kim

  5. Department of Farm Management, Kyungpook National University, Daegu, 41566, Korea

    Tae Kwon Son

  6. Apple Bio Co. Ltd, Daegu, 41566, Korea

    Tae Kwon Son

  7. BET Research Institute, Chung-Ang University, Anseong, 17546, Korea

    Sanghyun Lee

  8. Natural Product Institute of Science and Technology, Anseong, 17546, Korea

    Sanghyun Lee

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  1. Juree Kim
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Kim, J., Phung, H.M., Lee, S. et al. Anti-skin-aging effects of tissue-cultured mountain-grown ginseng and quantitative HPLC/ELSD analysis of major ginsenosides. J Nat Med 76, 811–820 (2022). https://doi.org/10.1007/s11418-022-01633-2

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