ELISA for the Detection of Marker Compound for Crop Fertilizer Use of Various Medicinal Crop Extracts using Bacterium - Collections

＜departmental bulletin paper＞
ELISA for the Detection of Marker Compound for Crop Fertilizer Use of Various Medicinal Crop Extracts using Bacterium

Creator	Creator Name SON, Tae–Kwon Affiliation Affiliation Name Department of Farm Management, College of Agriculture and Life Sciences, Kyungpook National University Affiliation Affiliation Name Biotron Application Center, Kyushu University
	Author PID K001590 Creator Name EGUCHI, Toshihiko 江口, 壽彦エグチ, トシヒコ Affiliation Affiliation Name Biotron Application Center, Kyushu University 生物環境利用推進センター
	Author PID 70037604 Creator Name SHOYAMA, Yukihiro 正山, 征洋ショウヤマ, ユキヒロ Affiliation Affiliation Name Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University Affiliation Affiliation Name Biotron Application Center, Kyushu University
	Author PID 30253470 Creator Name TANAKA, Hiroyuki 田中, 宏幸タナカ, ヒロユキ Affiliation Affiliation Name Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University Affiliation Affiliation Name Biotron Application Center, Kyushu University
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2019-02-28
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	64
Issue	1
First Page	27
Last Page	32
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/2231630

File	FileType	Size	Views	Description
p027	pdf	766 KB	511

PISSN	0023-6152
NCID	AA00697606
Record ID	2231630
Subject Terms	Crop fertilizer
	ginseng
	Bupleurum species
	ginsenoside
	saikosaponin
	ELISA
Notes	This study builds an easy and quick method of analysing the active ingredients in bio–fertilizers to ensure the uniformity of materials in bio–fertilizers that are made using ingredients that are cruder herbal materials or remnants after the primary medicinal crops are used as herbal materials. An ELISA was created using MAb, and it can substitute for HPLC in the analysis of saikosaponin a (SSa), ginsenoside (G– Rb1) and ginsenoside (G–Rg1), which are active ingredients of ginseng, and the ELISA can be used for various ginseng plants grown as typical medicinal crops. The concentration measurement ranges of G–Rb1, G–Rg1, and SSa are 12.5~250 ng/mL, 0.5~12.5 μg/mL, and 0.26~1.5 μg/mL, respectively.
Funding Information	Funder Name Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry Award Number 817022–3
Funding Information	Funder Name Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry Award Number 112044–3
Created Date	2019.04.15
Modified Date	2023.11.14