Abstract
Resistance to antibiotics is on the rise, and its indiscriminate usage has resulted in human and animal management constraints. In the research for an innovative treatment to diminish antimicrobial resistance, lactic acid bacteria (LAB) throw light on diminishing this problem in public health. As a result, this paper looked at the efficacy of LAB isolates and their active metabolites to combat pathogens, reduce antibiotic use in clinical settings, and explore the anticancer potential of 8 strains of LAB isolated from dairy products. Antifungal and antibacterial potential of LAB isolates against selected crop pathogenic fungi and food pathogenic bacteria had been estimated. Results revealed that all isolates exert antioxidant efficacy relating to DPPH, NO scavenging ability, reducing power, superoxide anion, hydroxyl radical, and anti-lipid peroxidation potential. Additionally, 12B isolate exert the highest anticancer upshot with IC50 values of 43.98 ± 0.4; 36.7 ± 0.6, 43.1 ± 0.8, and 35.1 ± 0.3 μg/ml, versus Caco-2, MCF-7, HepG-2, and PC3 cell lines respectively, whereas 13B isolate significantly had the highest selectivity index between peripheral blood mononuclear cells (PBMCs) and the tested human cancer cell lines compared to 5-fluorouracil. 13B was the most apoptosis-dependent death inducer for all human cancer cell lines besides exerting the lowest percentage of apoptosis against PBMCs suggesting its safety against PBMCs. The most promising strains 12B and 13B were identified by 16S rRNA sequencing as Lactiplantibacillus plantarum ESSG1 (MZ683194.1) and Lactiplantibacillus pentosus ESSG2 (MZ683195.1). LAB and their extracts are superb substitutive, safe, and efficient antimicrobial, antioxidant, and antitumor curative agents.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data availability
This paper offers all the data gathered or evaluated throughout this work.
References
Abdel Latef AAH, Kordrostami M, Zakir A, Zaki H, Saleh OM (2019) Eustress with H2O2 facilitates plant growth by improving tolerance to salt stress in two wheat cultivars. Plants 8(9):303. https://doi.org/10.3390/plants8090303
Ahn KB, Baik JE, Park OJ, Yun CH, Han SH (2018) Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans. PLoS ONE 13:e0192694. https://doi.org/10.1371/journal.pone.0192694
Albano RM, Mourao PA (1986) Isolation, fractionation, and preliminary characterization of a novel class of sulfated glycans from the tunic of Styelaplicata (Chordata tunicata). J BiolChem 261(2):758–765. https://doi.org/10.1038/s41396-021-01059-w
Altschul FS, Thomas LM, Alejandro AS, Jinghui Z, Zheng Z, Webb M (1997) David JL (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25(17):3389–3402. https://doi.org/10.1093/nar/25.17.3389
Amaretti A, di Nunzio M, Pompei A, Raimondi S, Rossi M, Bordoni A (2013) Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities. Appl Microbiol Biotechnol 97:809–817. https://doi.org/10.1007/s00253-012-4241-7
Asha GD (2012) Synergistic impact of Lactobacillus fermentum, Lactobacillus plantarum, and vincristine on 1,2- dimethylhydrazine-induced colorectal carcinogenesis in mice. Exp Ther Med 3:1049–1054. https://doi.org/10.3892/etm.2012.536
Baffoni L, Gaggìa F, Di Gioia D, Biavati B (2012) Role of intestinal microbiota in colon cancer prevention March 2012. Annals of microbiology 62(1):15–30. https://doi.org/10.1007/s13213-011-0306-6
Baldwin C, Millette M, Oth D, Ruiz MT, Luquet FM, Lacroix M (2010) Probiotic Lactobacillus acidophilus and L. casei mix sensitize colorectal tumoral cells to 5-fuorouracilinduced apoptosis. Nutr Cancer 62:371–378. https://doi.org/10.1080/01635580903407197
Barrow GI, Feltham RKA (eds) (1993) Cowan and Steel’s manual for the identification of medical bacteria, 3rd edn. Cambridge University Press, Cambridge
Beck BR, Park GS, Lee YH, Im S, Jeong DY, Kang J (2019) Whole genome analysis of Lactobacillus plantarum strains isolated from kimchi and determination of probiotic properties to treat mucosal infections by Candida albicans and Gardnerella vaginalis. Front Microbiol 10:433. https://doi.org/10.3389/fmicb.2019.00433
Bédard F, Biron E (2018) Recent Progress in the chemical synthesis of class II and S-glycosylated bacteriocins. Front Microbiol 9:1048. https://doi.org/10.3389/fmicb.2018.01048
Bergamo A, Dyson PJ, Sava G (2018) The mechanism of tumor cell death by metal-based anticancer drugs is not only a matter of DNA interactions. Coord Chem Rev J 360:17–33. https://doi.org/10.1016/j.ccr.2018.01.009
Biasi F, Astegiano M, Maina M, Leonarduzzi G, Poli G (2011) Polyphenol supplementation as a complementary medicinal approach to treating inflammatory bowel disease. Curr Med Chem 18:4851–4865. https://doi.org/10.2174/092986711797535263
Bikheet MM, Darwish AM, Hassanein KA, Shalaby SI (2015) Lactic acid bacteria with potential to eliminate fungal spoilage in cheeses) proceeding the 2nd Minia International Conference "Agriculture and Irrigation in Nile Basin Countries”32-25 March, Minia, Egypt. 312-317. https://doi.org/10.21203/rs.3.rs-313107/v1
Borges A, Silva M, Recchia GH, de Queiroz-Silva JR, Bressan EA, Veasey EA (2009) CTAB methods for extraction of sweet potato for microsatellite analysis. Sci Agric (Piracicaba, Braz) 66(4):529
Brenner DM, Chey WD (2009) Bifidobacterium infantes 35624: a novel probiotic for the treatment of irritable bowel syndrome. Rev Gastroenterol Disorder 9:7–15
Chen D, Huang JF, Liu K, Zhang LQ, Yang Z et al (2014) BRAFV600E mutation and its association with clinicopathological features of colorectal cancer: a systematic review and meta-analysis. PLoS ONE 9(3):e90607. https://doi.org/10.1371/journal.pone.0090607
Cowan ST (1974) Cowan and Steel’s manual for the identification of medical bacteria, 2nd edn. Cambridge University Press, Cambridge
de Man JC, Rogosa M, Sharpe EM (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135. https://doi.org/10.1111/j.1365-2672.1960.tb00188.x
Delesa DA (2017) Overview of anticancer activity of lactic acid bacteria. Int J Adv Res Biol Sci. 4(12):166. https://doi.org/10.22192/ijarbs
Dinev T, Beev G, Tzanova M, Denev S, Dermendzhieva D, Stoyanova A (2018) Antimicrobial activity of Lactobacillus plantarum against pathogenic and food spoilage microorganisms: a review. Bulg J Vet Med. 21:253–268. https://doi.org/10.15547/bjvm.1084
Ding C, Tang W, Fan X (2018) Wu G (2018) Onco targets and therapy dove press intestinal microbiota: a novel perspective in colorectal cancer biotherapeutics. Oncotargets Ther 11:4797–4810. https://doi.org/10.2147/OTT.S170626
Dowarah R, Verma A, Agarwal N (2017) The use of Lactobacillus as an alternative of antibiotic growth promoters in pigs: a review. Anim Nutr 3:1–6. https://doi.org/10.1016/j.aninu.2016.11.002
Ebrahimzadeh M, Nabavi S, Nabavi S, Bahramian F, Bekhradnia R (2008) Antioxidant and free radical scavenging activity. Pak J Pharm Sci. 23:29–34
El-Ansari MA, Ibrahim LF, Sharaf M (2019) Natural phenolics: a source of anticancer agents. Egypt Pharma ceut J. 18:1–7. https://doi.org/10.4103/epj.epj_43_18
El-Deeb NM, Yassin AM, Al-Madboly LA, El-Hawiet A (2018) A novel purified Lactobacillus acidophilus 20079 exopolysaccharide, LA-EPS-20079, molecularly regulates both apoptotic and NF-κB inflammatory pathways in human colon cancer. Microb Cell Factories 17(1):29. https://doi.org/10.1186/s12934-018-0877-z
Fathy RM, Mahfouz AY (2021) Eco-friendly graphene oxide-based magnesium oxide nanocomposite synthesis using fungal fermented by-products and gamma rays for outstanding antimicrobial, antioxidant, and anticancer activities. J Nanostruct Chem 11:301–321. https://doi.org/10.1007/s40097-020-00369-3
Fontana M, Mosca L, Rosei MA (2001) Interaction of enkephalins with oxy radicals. Biochem. Pharmacol 61:1253–1257. https://doi.org/10.1016/s0006-2952(01)00565-2
Garcia-Gonzalez N, Battista N, Prete R, Corsetti A (2021) Health-promoting role of Lactiplantibacillus plantarum isolated from fermented foods. Microorganisms 9(2):349. https://doi.org/10.3390/microorganisms9020349
Garnier L, Valence F, Pawtowski A, Auhustsinava-Galerne L, Frotté N, Baroncelli R et al (2016) Diversity of spoilage fungi associated with various French dairy products. Int J Food Microbiol 241:191–197. https://doi.org/10.1016/j.ijfoodmicro.2016.10.026
Georgieva R, Yocheva L, Tserovska L, Zhelezova G, Stefanova N, Atanasova A et al (2015) Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol Biotechnol Equip 29:84–91. https://doi.org/10.1080/13102818.2014.987450
Gini TG, Jeya Jothi G (2018) Column chromatography and HPLC analysis of phenolic compounds in the fractions of Salvinia molesta mitchell. Egyptian Journal of Basic and Applied Sciences 5(3):197–203. https://doi.org/10.1016/j.ejbas.2018.05.010
Ho SC, Tang YL, Lin SM, Liew YF (2010) Evaluation of peroxynitrite-scavenging capacities of several commonly used fresh spices. Food Chem 119(3):1102–1107. https://doi.org/10.1016/j.foodchem.2009.08.020
Houghton PJ, Zarka R, de la Heras B, Hoult JRS (1995) Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 61:33–36. https://doi.org/10.1055/s-2006-957994
Inglin RC, Stevens MJ, Meile L, Lacroix C, Meile L (2015) High throughput screening assays for antibacterial and antifungal activities of Lactobacillus species. J Microbiol Methods 114:26–29. https://doi.org/10.1016/j.mimet.2015.04.011
Jamalifar H, Rahimi H, Samadi N, Shahverdi A, Sharifian Z, Hosseini F, Eslahi H, Fazeli M (2011) Antimicrobial activity of different Lactobacillus species against multi- drug resistant clinical isolates of Pseudomonas aeruginosa. Iran J Microbiol 3(1):21–5
Kahouli I, Malhotra M, Westfall S, Alaoui-Jamali MA, Prakash S (2017) Design and validation of an orally administrated active L. fermentum - L. acidophilus probiotic formulation using colorectal cancer Apc Min/+ mouse model. Appl Microbiol Biotechnol 101:1999–2019. https://doi.org/10.1007/s00253-016-7885-x
Kang MS, Lim HS, Oh JS, Lim YJ, Wuertz-Kozak K, Harro JM, et al. (2017) Antimicrobial activity of Lactobacillus salivarius and Lactobacillus fermentum against Staphylococcus aureus. Pathog Dis 75(2). https://doi.org/10.1093/femspd/ftx009.
Kang CH, Kim Y, Han SH, Kim JS, Paek NS, So JS (2018) In vitro probiotic properties of vaginal Lactobacillus fermentum MG901 and Lactobacillus plantarum MG989 against Candida albicans. Eur J Obstet Gynecol Reprod Biol 228:232–237. https://doi.org/10.1016/j.ejogrb.2018.07.005
Karska-Wysockib B, Bazoa M, Smoragiewicza W (2010) Antibacterial activity of Lactobacillus acidophilus and Lactobacillus casei against methicillin-resistant Staphylococcus aureus (MRSA). Microbiol Res 165:674–686. https://doi.org/10.1016/j.micres.2009.11.008
Kim JS (2012) Comparison of antioxidant properties of water and ethanol extracts obtained from dried boxthorn (Lycium chinensis) fruit. Food Nutr Sci 3:1307–1320. https://doi.org/10.4236/fns.2012.39173
Kumar M, Dhaka P, Vijay D, Vergis J, Mohan V, Kumar A et al (2016) Antimicrobial effects of Lactobacillus plantarum and Lactobacillus acidophilus against multidrug-resistant enteroaggregative Escherichia coli. Int J Antimicrob Agents 48:265–270. https://doi.org/10.1016/j.ijantimicag.2016.05.014
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetic analysis across computing platform. Mol Biol Evol 35(6):1547–1549. https://doi.org/10.1093/molbev/msy096
Larkin MA, Blackshields G, Brown NP et al (2007) Clustal W and Clustal X Version 2.0. Bioinformatics 23:2947–2948. https://doi.org/10.1093/bioinformatics/btm404
Lee JW, Shin JG, Kim EH, Kang HE, Yim IB, Kim JY, Joo H, Woo HJ (2004) Immunomodulatory and Antitumor Effects in Vivo by the Cytoplasmic Fraction of Lactobacillus Casei and Bifidobacterium Longum. J Vet Sci 5(1):41–48
Lee JE, Lee J, Kim JH, Cho N, Lee SH, Park SB, Koh B, Kang D, Kim S, Yoo HM (2019) Characterization of the anti-cancer activity of the probiotic bacterium Lactobacillus fermentum using 2D vs. 3D culture in colorectal cancer cells. Biomolecules 9(10):557. https://doi.org/10.3390/biom9100557
Li S, Zhao Y, Zhang L, Zhang X, Huang L, Li D et al (2012) Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods. Food Chem 135:1914–1919. https://doi.org/10.1016/j.foodchem.2012.06.048
Lin MY, Yen CL (1999) Antioxidative ability of lactic acid bacteria. J Agric Food Chem 47:1460–1466. https://doi.org/10.1021/jf981149l
Lin X, Yongjun X, Guangqiang W, Yijin Y, Zhiqiang X, Fang L, Wei Z (2018) Lianzhong A (2018) Lactic acid bacteria with antioxidant activities alleviating oxidized oil induced hepatic injury in mice. Front Microbiol 9:2684. https://doi.org/10.3389/fmicb.2018.02684
Liu W, Pang H, Zhang H, Cai Y (2014) Biodiversity of lactic acid bacteria. In Lactic acid bacteria; J.B. Metzler, Ed.; Springer: Berlin/Heidelberg, Germany, 103–203.
Maldonado NC, de Ruiz CS, Otero MC, Sesma F, Nader- Macías ME (2012) Lactic acid bacteria isolated from young calves—characterization and potential as probiotics. Res Vet Sci 92:342–349. https://doi.org/10.1016/j.rvsc.2011.03.017
Salas ML, Anne T, Mathilde L, Gilles G, Marielle H, Manon C, Lê Sébastien Jérôme M, Florence V, Emmanuel C (2018) Antifungal activity of lactic acid bacteria combinations in dairy mimicking models and their potential as bioprotective cultures in pilot scale applications. Front Microbiol 9:1787. https://doi.org/10.3389/fmicb.2018.01787
Martinez-Canavate A, Sierra S, Lara-Villoslada F, Romero J, Maldonado J, Boza J, Xaus J, Olivares M (2009) A probiotic dairy product containing L. gasseri CECT5714 and L. coryniformis CECT5711 induces immunological changes in children suffering from allergy. Pediatric Allergy Immunol 20:592–600. https://doi.org/10.1111/j.1399-3038.2008.00833.x
Maurício E, Rosado C, Duarte MP, Verissimo J, Bom S, Vasconcelos L (2017) Efficiency of nisin as preservative in cosmetics and topical products. Cosmetics 4(4):41. https://doi.org/10.3390/cosmetics4040041
McFarland LV (2015) Probiotics for the primary and secondary prevention of C. difficile infections: a meta-analysis and systematic review. Antibiotics 4:160–178. https://doi.org/10.3390/antibiotics4020160
McNicholl AG, Molina-Infante J, Lucendo AJ, Calleja JL, Pérez-Aisa Á, Modolell I, Aldeguer X, Calafat M, Comino L (2018) Ramas M (2018) Probiotic supplementation with Lactobacillus plantarum and Pediococcus acidilactici for Helicobacter pylori therapy: a randomized, double-blind, placebo-controlled rial. Helicobacter 23(5):e12529. https://doi.org/10.1111/hel.12529
Mende S, Rohm H, Jaros D (2016) Influence of exopolysaccharides on the structure, texture, stability and sensory properties of yoghurt and related products. Int Dairy J 52:57–71. https://doi.org/10.1016/j.idairyj.2015.08.002
Mendoza L (2019) Potential effect of probiotics in the treatment of breast cancer. Oncol Rev 13(2):422. https://doi.org/10.4081/oncol.2019.422
Mirnejad R, Vahdati AR, Rashidiani J, Erfani M, Piranfar V (2013) The antimicrobial effect of Lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of Shigella sonnei and Shigella flexneri in vitro. Iran Red Crescent Med J 15:122–126. https://doi.org/10.5812/ircmj.7454
Mokoena MP (2017) Lactic acid bacteria and their bacteriocins: classification, biosynthesis and applications against uropathogens: a mini-review. Molecules 22(8):1255. https://doi.org/10.3390/molecules22081255
Molaae N, Mosayebi G, Pishdadian A, Ejtehadifar M, Ganji A (2017) Evaluating the proliferation of human peripheral blood mononuclear cells using MTT assay. Int J Basic Sci Med 2(1):25–28. https://doi.org/10.15171/ijbsm.2017.06
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1):55–63. https://doi.org/10.1016/0022-1759(83)90303-4
Moura FA, de Andrade KQ, Dos Santos JCF, Araújo ORP, Goulart MOF (2015) Antioxidant therapy for treatment of inflammatory bowel disease: does it work? Redox Biol 6:617–639. https://doi.org/10.1016/j.redox.2015.10.006
Nami Y, Abdullah NF, Haghshenas B, Radiah D, Rosli R, Khosroushahi AY (2014) Assessment of probiotic potential and anticancer activity of newly isolated vaginal bacterium Lactobacillus plantarum 5BL. Microbiol Immunol 58:492–502. https://doi.org/10.1111/1348-0421.12175
Nguyen T, Laosinwattana C, Teerarak M, Pilasombut K (2017) Potential antioxidant and lipid peroxidation inhibition of Phyllanthus acidus leaf extract in minced pork. Asian Australas J Anim Sci 30(9):1323–1331. https://doi.org/10.5713/ajas.17.0036
Onbas T, Osmanagaoglu O, Kiran F (2019) Potential properties of Lactobacillus plantarum F-10 as a bio-control strategy for wound infections. Probiotics Antimicrob Proteins 11:1110–1123. https://doi.org/10.1007/s12602-018-9486-8
Oyaizu M (1986) Studies on product of browning reaction prepared from glucose amine. Jpn J Nutri 44:307–315. https://doi.org/10.5264/eiyogakuzashi.44.307
Pan D, Mei X (2010) Antioxidant activity of an exopolysaccharide purified from Lactococcus lactis subsp. lactis 12. Carbohyd Polym 80:908–914. https://doi.org/10.1016/j.carbpol.2010.01.005
Pascual L, Ruiz F, Giordano W, Barberis IL (2010) Vaginal colonization and activity of the probiotic bacterium Lactobacillus fermentum L23 in a murine model of vaginal tract infection. J Med Microbiol 59:360–364. https://doi.org/10.1099/jmm.0.012583-0
Pavithra K, Vadivukkarasi S (2015) Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Sci Human Wellness 4:42–46. https://doi.org/10.1016/j.fshw.2015.02.001
Perpetuini G, Prete R, Garcia-Gonzalez N, Khairul Alam M, Corsetti A (2020) Table olives more than a fermented food. Foods 9:178. https://doi.org/10.3390/foods9020178
Reed JC (2018) Bcl-2 on the brink of breakthroughs in cancer treatment. Nat Publ Gr 25:3–6. https://doi.org/10.1038/cdd.2017.188
Riedl SJ, Shi Y (2004) Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol 5:897–907. https://doi.org/10.1038/nrm1496
Rodriguez E, Arques JL, Rodrıguez R, Peiroten A, Landete JM, Medina M (2012) Antimicrobial properties of probiotic strains isolated from breast-fed infants. J Funct Foods 4:542–551. https://doi.org/10.1016/j.jff.2012.02.015
Russo P, Arena MP, Fiocco D, Capozzi V, Drider D, Spano G (2017) Lactobacillus plantarum with broad antifungal activity: a promising approach to increase safety and shelf-life of cereal-based products. Int J Food Microbiol 247:48–54. https://doi.org/10.1016/j.ijfoodmicro.2016.04.027
Ryan PM, Ross RP, Fitzgerald GF (2015) Sugar-coated: exopolysaccharide producing lactic acid bacteria for food and human health applications. Food Funct 6:679–693. https://doi.org/10.1039/c4fo00529e
Seddik HA, Bendali F, Gancel F, Fliss I, Spano G, Drider D (2017) Lactobacillus plantarum and its probiotic and food potentialities. Probiotics & Antimicro Prot 9:111–122. https://doi.org/10.1007/s12602-017-9264-z
Shipkova M, Wieland E (2012) Surface markers of lymphocyte activation and markers of cell proliferation. Clin Chim Acta 413(17–18):1338–1349. https://doi.org/10.1016/j.cca.2011.11.006
Silva CCG, Silva SPM, Ribeiro SC (2018) Application of bacteriocins and protective cultures in dairy food preservation. Front Microbiol 9:594. https://doi.org/10.3389/fmicb.2018.00594
Smirnoff N, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28(4):1057–1060. https://doi.org/10.1016/0031-9422(89)80182-7
Soltani Samira, Riadh Hammami, Paul D Cotter, Sylvie Rebuffat, Laila Ben Said, Hélène Gaudreau, François Bédard, Eric Biron, Djamel Drider, Ismail Fliss, (2021) Bacteriocins as a new generation of antimicrobials: toxicity aspects and regulations, FEMS Microbiology Reviews, 45(1): January, fuaa039, https://doi.org/10.1093/femsre/fuaa039
Taga MS et al (1984) Chia seeds as a source of natural lipid antioxidants. J Am Oil Chem. Soc 61:928–931. https://doi.org/10.1007/BF02542169
Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
Tukenmez U, Aktas B, Aslim B, Yavuz S (2019) The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro. Sci Rep 9:8268. https://doi.org/10.1038/s41598-019-44753-8
Vieco-Saiz N, Belguesmia Y, Raspoet R, Auclair E, Gancel F, Kempf I, Drider D (2019) Benefits and inputs from lactic acid bacteria and their bacteriocins as alternatives to antibiotic growth promoters during food-animal production. Front Microbiol 10:57. https://doi.org/10.3389/fmicb.2019.00057
Wang SM, Zhang L, Fan R, Han Xue, Yi Hua-Xi, Zhang L, Xue C, Li H, Yan- Zhang H, Shigwedha N (2014) Induction of HT-29 cells apoptosis by lactobacilli isolated from fermented products. Res Microbiol 165(3):202–14. https://doi.org/10.1016/j.resmic.2014.02.004
Williams ST, Sharpe ME, Holt JG (Editors) (1989) Bergey’s manual of systematic bacteriology, 1st Ed., Vol. 4, The Williams & Wilkins Co., Baltimore
Yazdi MH, Soltan Dallal MM, Hassan ZM (2010) Oral administration of Lactobacillus acidophilus induces IL-12 production in spleen cell culture of BALB/c mice bearing transplanted breast tumour. Br J Nutr 104:227–232. https://doi.org/10.1017/S0007114510000516
Yi ZJ, Fu YR, Li M, Gao KS, Zhang XG (2009) Effect of LTA isolated from Bifidobacteria on D-galactose-induced aging. Exp Gerontol 44:760–765. https://doi.org/10.1016/j.exger.2009.08.011
Zarate G, Nader-Macias ME (2006) Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules. Proc Biochem 41:1779–1785. https://doi.org/10.1016/j.procbio.2006.03.024
Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64(4):555–559. https://doi.org/10.1016/S0308-8146(98)00102-2
Funding
The authors acknowledge the financial support of Taif University Researchers Supporting Project number (TURSP-2020/82), Taif University, Taif, Saudi Arabia.
Author information
Authors and Affiliations
Contributions
Designed the experiments: S.A, E.S.A, and M. M.A Performed the experiments S.A, E.S.A, and M. M.A, A.Y.M.; analyzed the data: A.Y.M and G.E.D.; writing, original draft preparation: A.Y.M, G.E.D, A.E, N.A.B, A.A.A.; writing, review, and editing (major contributor in writing the manuscript): S.A, M.M.A, A.Y.M, and G.E.D.; funding acquisition: A.E.; all authors have read and agreed to the published version of the manuscript.
Corresponding author
Ethics declarations
Research involving human participants and/or animals
There are no experiments on person or animal bodies in this publication.
Conflict of interest
The authors declare no competing interests.
Additional information
Responsible Editor: Lotfi Aleya
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Abd Ellatif, S.A., Bouqellah, N.A., Abu-Serie, M.M. et al. Assessment of probiotic efficacy and anticancer activities of Lactiplantibacillus plantarum ESSG1 (MZ683194.1) and Lactiplantibacillus pentosus ESSG2 (MZ683195.1) isolated from dairy products. Environ Sci Pollut Res 29, 39684–39701 (2022). https://doi.org/10.1007/s11356-022-18537-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-022-18537-z