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An improved micropropagation of Eclipta alba by in vitro priming with chlorocholine chloride

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Abstract

An efficient method of micropropagation for Eclipta alba from young nodal axils of shoot tip explants has been developed by giving special attention to ‘priming’ in vitro plantlets in view of increasing their hardening ability after transplantation ex vitro. Among 3 cytokinins—BAP, kinetin and TDZ, BAP was found most effective in inducing and proliferating adventitious shoots. The highest frequency of responding explants (100%) and maximum number of shoots (23.0) per explant were obtained after 60 days culture on MS medium containing 8.8 μM BAP. Cent percent shoots developed roots directly from shoot base when transferred to growth regulator-free MS medium. For priming E. alba microshoots, 6.3 μM of chlorocholine chloride (CCC) was found most effective. The major changes observed in 30 days old treated shoots were, production of increased number of root, elevation of chlorophyll level in leaves and increase in plant biomass. Furthermore, arrested undesirable shoot elongation made the plants sturdier and more suitable for acclimatization. The primed micropropagated E. alba plants were healthy and survived by higher frequency (100%) in soil in comparison to the non-treated plants (84% survival).

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Abbreviations

BAP:

6-Benzylaminopurine

TDZ:

Thiadiazuron

CCC:

Chlorocholine chloride

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Acknowledgements

Authors gratefully acknowledge the University Grants Commission, Govt. of India, New Delhi for financial support in conducting the research. We also thank Mr. Jadab Ghosh, Dept. of Botany, Bose Institute for providing technical assistance.

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  1. Department of Botany, Bose Institute, 93/1, A.P.C. Road, Kolkata, 700 009, West Bengal, India

    Avik Ray & Sabita Bhattacharya

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  1. Avik Ray
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  2. Sabita Bhattacharya
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Correspondence to Avik Ray.

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Ray, A., Bhattacharya, S. An improved micropropagation of Eclipta alba by in vitro priming with chlorocholine chloride. Plant Cell Tiss Organ Cult 92, 315–319 (2008). https://doi.org/10.1007/s11240-007-9328-y

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  • DOI: https://doi.org/10.1007/s11240-007-9328-y

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