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Phosphoproteomics of cold stress-responsive mechanisms in Rhododendron chrysanthum

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Abstract

Background

As an alpine plant, Rhododendron chrysanthum (R. chrysanthum) has evolved cold resistance mechanisms and become a valuable plant resource with the responsive mechanism of cold stress.

Methods and results

We adopt the phosphoproteomic and proteomic analysis combining with physiological measurement to illustrate the responsive mechanism of R. chrysanthum seedling under cold (4 °C) stress. After chilling for 12 h, 350 significantly changed proteins and 274 significantly changed phosphoproteins were detected. Clusters of Orthologous Groups (COG) analysis showed that significantly changed phosphoproteins and proteins indicated cold changed energy production and conversion and signal transduction.

Conclusions

The results indicated photosynthesis was inhibited under cold stress, but cold induced calcium-mediated signaling, reactive oxygen species (ROS) homeostasis and other transcription regulation factors could protect plants from the destruction caused by cold stress. These data provide the insight to the cold stress response and defense mechanisms of R. chrysanthum leaves at the phosphoproteome level.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

ALDH:

NAD(P) + −  dependent aldehyde dehydrogenases

CaM-BP:

CaM-binding protein

CaM:

Calcium

CAT:

Catalase

COG:

Clusters of Orthologous Groups of proteins

MAP3K:

Epsilon protein kinase 1

NDPK2:

Nucleoside diphosphate kinase 2 protein kinase

OEC:

Photosystem II oxygen evolving complex

POD:

Peroxidase

SOD:

Superoxide dismutase

TMT Labeling:

Tandem mass tags labeling

STP:

Serine/threonine-protein kinase

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Funding

This work was mainly supported by the National Natural Science Foundation of China (31070224) and the Science and Technology Department of Jilin Province (20130206059NY).

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Authors and Affiliations

  1. Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping, 136000, China

    Yunbo Liu, Hang Fan, Jiawei Dong, Jianyu Chen, Hongwei Xu & Xiaofu Zhou

Authors
  1. Yunbo Liu
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  2. Hang Fan
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  3. Jiawei Dong
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  4. Jianyu Chen
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  5. Hongwei Xu
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  6. Xiaofu Zhou
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Contributions

XZ and HX designed the research; YL and HF prepared the plant materials for sequencing. YL carried out bioinformatics analysis of data; YL, HF and JC performed the experiments and statistical analyses; YL and JD collected data and researched literature, YL interpreted the data and wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hongwei Xu or Xiaofu Zhou.

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Liu, Y., Fan, H., Dong, J. et al. Phosphoproteomics of cold stress-responsive mechanisms in Rhododendron chrysanthum. Mol Biol Rep 49, 303–312 (2022). https://doi.org/10.1007/s11033-021-06874-0

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