Abstract
It has been reported that F1 LA hybrids could produce a small number of functional 2n eggs regardless of their male sterility, however, there are very little similar phenomena reported in OA, LO, or OT hybrids, though there are many allotriploid cultivars in flower markets, involving distant hybrids. To investigate the occurrence of 2n eggs in distant lily F1 hybrids, a diploid OT hybrid was analyzed for its meiosis and progenies using conventional method and genomic in situ hybridization. The results showed that the diploid OT hybrid had abnormal meiosis and was highly male sterile, however, as seed parent, its progenies were triploid or near triploid, implying that it could produce a small number of functional 2n eggs. The genome compositions of the triploids indicated that the functional 2n eggs were predominantly from first division restitution mechanism, though they were variable due to the differences among their intergenomic recombinant chromosomes. In addition, all the progenies grew vigorously and variously, especially for their flower color, demonstrating that the polyploidization and variable intergenomic recombinations were important for breeding lily cultivars from BC1 progenies. It is concluded, in Lilium breeding, that the occurrence of a small number of functional 2n eggs in F1 distant hybrids is quite common, and their triploid progenies are the main origin of allotriploid lily cultivars.
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This work was supported by the National Natural Science Foundation of China for financial supports (Nos. 31572154 and 31460527).
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YL did most of experiments and preliminary manuscript. LZ read the manuscript and gave some comments. YS did some parts of experiments. SZ did conceptualization, methodology, and manuscript writing and correction.
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Liu, Y., Zhang, L., Sun, Y. et al. The common occurrence of 2n eggs by lily F1 distant hybrids and its significance on lily breeding: a case of analyzing OT hybrids. Euphytica 217, 204 (2021). https://doi.org/10.1007/s10681-021-02935-4
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DOI: https://doi.org/10.1007/s10681-021-02935-4