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Variation in GmAOS1 promoter is associated with soybean defense against insect attack

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Abstract

Soybean (Glycine max L.) displays considerable variation in its resistance to insects. Characterization and application of insect resistance genes in soybean germplasm may be a sustainable and economical approach to soybean production. In this study, we demonstrated that the gene encoding allene oxide synthase (GmAOS1) was associated with soybean defense against the common cutworm (CCW, Spodoptera litura Fabricius) attack through gene expression analysis and association analysis. The expression of GmAOS1 in stems and leaves of resistant accessions was higher than that of susceptible accessions in the undamaged soybean plant. After CCW feeding, the transcript levels of GmAOS1 increased and reached their maximal values sooner in the leaves of resistant accession than they did in susceptible accession. The genomic sequence of GmAOS1 was re-sequenced and aligned in 184 soybean accessions. Forty-three SNPs/Indels were found in its promoter region and only one single nucleotide polymorphism was found in its coding region. Association analysis showed that GmAOS1 promoter polymorphisms were significantly associated with three soybean defense indexes. GmAOS1_19 was the most resistant allele associated with soybean resistance to CCW. Two elements (TATCCAT/C motif and Box II-like sequence) located at genomic regions upstream of GmAOS1_21 were significantly associated with compensatory regrowth of soybean plants. Gene expression analysis indicated that the accessions containing different GmAOS1 promoters and with different CCW resistance grades showed different expression levels of GmAOS1. Our data indicate that variation in GmAOS1 promoter is associated with soybean defense against CCW attack.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31201230) and the Natural Science Foundation of Jiangsu Province of China (BK2012768).

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  1. Soybean Research Institute, Nanjing Agricultural University, National Center for Soybean Improvement, Ministry of Agriculture, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, 210095, Jiangsu, China

    Hui Wang, Zhongjie Gao, Hailun Liu, Juanjuan Wu & Deyue Yu

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  1. Hui Wang
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  2. Zhongjie Gao
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Correspondence to Deyue Yu.

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Wang, H., Gao, Z., Liu, H. et al. Variation in GmAOS1 promoter is associated with soybean defense against insect attack. Euphytica 196, 365–374 (2014). https://doi.org/10.1007/s10681-013-1038-4

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