biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 49:277-280, 2005 | DOI: 10.1007/s10535-005-7280-4

A new medium formulation for in vitro rooting of carob tree based on leaf macronutrients concentrations

S. Gonçalves1, P. J. Correia1, M. A. Martins-Loução2, A. Romano1,*
1 Faculdade de Engenharia de Recursos Naturais, Universidade do Algarve, Campus de Gambelas, Faro, Portugal
2 Faculdade de Ciências, Universidade de Lisboa, Campo Grande, Lisboa, Portugal

Experiments were performed to optimize the macronutrients concentrations for in vitro rooting of Ceratonia siliqua micropropagated shoots. Several dilutions of Murashige and Skoog (MS) medium were tested: full-strength MS, half-strength MS (1/2MS), and 1/2MS + full N. The frequency of in vitro rooting was enhanced when the 1/2MS was used (50 % rooted shoots). Mature leaves from 20 - 30 year-old carob trees and from 2 year-old micropropagated plants were collected and the concentrations of macronutrients (N, P, K, Ca, Mg) assessed. Based on the mineral composition of the leaves a new medium was formulated and compared with the previous ones showing an increment of the rooting frequency to 80 %. Moreover, shoots rooted in the new medium did not show symptoms of apical necrosis that occurred in the other tested media.

Keywords: Ceratonia siliqua; culture medium; micropropagation; nutrient medium
Subjects: adventitious shoot, root; auxins; carob tree, new nutrient medium; Ceratonia siliqua; cytokinins; in vitro culture, adventitious shoots and roots; in vitro culture, mass propagation; nutrient medium MS (Murashige and Skoog)

Received: March 29, 2004; Accepted: December 14, 2004; Published: June 1, 2005  Show citation

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Gonçalves, S., Correia, P.J., Martins-Loução, M.A., & Romano, A. (2005). A new medium formulation for in vitro rooting of carob tree based on leaf macronutrients concentrations. Biologia plantarum, 49(2), 277-280. doi: 10.1007/s10535-005-7280-4
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