Summary
Mineral deficiency symptoms were observed in leaves of yellow passionfruit plantlets grown in MS medium (Murashige and Skoog, 1962) with 1.0 mg l−1 (3.0 μM) gibberellic acid. Initially, leaves showed interveinal chlorosis, followed by bleaching of the leaves and retarded growth. Leaf mineral analysis was done and compared to mineral requirements suggested for passionfruit in the literature. Several modifications were made to the inorganic composition of MS medium, according to mineral deficiencies, mainly of Fe and Ca, and possible toxicity of Cl. The concentration of the elements in the new medium (MSM) was based on the mineral composition of leaves of healthy plants. The chemical equilibrium was checked using the software Geochem (Sposito and Mattigod, 1980) and final adjustments were made to ensure good availability of nutrients. To test the efficiency of the modified medium nodal segments were cultured in both MS and MSM supplemented with 3.0 mg l−1 (13.3 μM) 6-benzyladenine. After three subcultures mineral analysis of the leaves was done. Severe mineral deficiency was observed on the leaves of plantlets cultured in MS, while plantlets cultivated in MSM had green leaves. A comparison of the mineral analysis of plantlets in both media showed a fairly large increase in Ca, Cu, Fe, Mg and S and decrease in levels of B and Cl in plantlets cultivated in MSM. A slight increase or decrease in other elements was also observed. Subculture of the chlorotic plantlets into MSM showed that the visual symptoms of mineral deficiency disappeared in 2–4 wk.
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References
Bould, C.; Hewitt, E.J.; Needham, P. Diagnosis of mineral disorders in plants: principles. London: HMSO; 1983: Vol. 1. 170pp.
Correia, D. Otimização da fase de multiplicação de gemas in vitro de Eucalyptus spp. Mestrado, Escola Superior de Agricultura Luiz de Queiroz. Universidade de São Paulo: Piracicaba; 1992:109.
Dornelas, M.C.; Vieira, M.L. Tissue culture studies on species of Passiflora. Plant Cell Tiss. Organ Cult. 36:211–217; 1994.
Faria, J.L.C.; Segura, J. In vitro control of adventitious bud differentiation by inorganic medium components and silver thiosulfate in explants of Passiflora edulis f. flavicarpa. In Vitro Cell. Dev. Biol. Plant 33:209–212; 1997.
Kantharajah, A.S.; Dodd, W.A. In vitro micropropagation of Passiflora edulis (purple passionfruit). Ann. Bot. 63:337–339; 1990.
Kawata, K.; Ushida, C.; Kawai, F.; Kanamori, M.; Kuriyama, A. Micropropagation of passionfruit from subcultured multiple shoot primordia. J. Plant Physiol. 147:281–284; 1995.
Malavolta, E.; Vitti, G.C.; Oliveira, S.A. Avaliação do estado nutricional das plantas: princípios e aplicações. 2nd edn. Piracicaba: POTAFOS; 1997;319pp.
Manders, G.; Otoni, W.C.; D'Utra Vaz, F.B.; Blackhall, N.W.; Power, J.B.: Davey, M.R. Transformation of passionfruit (Passiflora edulis f. flavicarpa Degener.) using Agrobacterium tumefaciens. Plant Cell Rep. 13:697–702; 1994.
Morales-Abanto, A.; Müller, L.E. Alteracionts producidas en el maracuyá (Passiflora edulis Sims) por deficiencias de manganeso, hierro, boro y zinc. Turrialba 27:163–168: 1997a.
Morales-Abanto, A.; Müller, L.E. Alteraciones producidas en el maracuyá (Passiflora edulis Sims) por deficiencias de magnesio, calcio y azufre. Turrialba 27:221–225; 1977b.
Moran Robles, M.J. Multiplication végétative, in vitro, des bourgeons axillares de Passiflora edulis var. flavicarpa Degener et de P. mollissima Bailey. Fruits 33:693–699; 1978.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15:473–497; 1962.
Otoni, W.C.; Casali, V.W.D.; Cecon, P.R.; Davey, M.R.; Power, J.B. Regeneração de plantas de maracujazeiro (Passiflora coccinea Aubl.) a partir de protoplastos derivados de mesófilo. Rev. Ceres 42:461–468; 1995.
Pedroso, M.C.; Pais, M.S. Factors controlling somatic embryogenesis. Plant Cell Tiss. Organ Cult. 43:147–154; 1995.
Reuter, D.J.; Robinson, J.B. Plant analysis: an interpretation manual. 2nd edn. Collingwood: CSIRO; 1997:572pp.
Rodriguez, A.P.M.; Wetzstein, H.Y. A morphological and histological comparison of the initiation and development of pecan (Carya illinoinensis) somatic embryogenic cultures induced with naphthaleneacetic acid or 2,4-dichlorophenoxyacetic acid. Protoplasma 204:71–83; 1998.
Ruggiero, C.; São José, A.R.; Volpe, C.A.; Oliveira, J.C.; Durigan, J.F.; Baumgartner, J.G.; Silva, J.R.; Nakamura, K.; Ferreira, M.E.; Kavati, R.; Pereira, V.P. Maracujá para exportação: aspectos técnicos da produção. Brasília: EMBRAPA-SPI; 1996:64pp.
Sposito, G.; Mattigod, S.V. Geochem: a computer program for the calculation of chemical equilibria in soil solutions and other material water systems. Riverside: Kearney Foundation of Soil Science, University of California; 1980:107pp.
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Monteiro, A.C.B.d.A., Higashi, E.N., Gonçalves, A.N. et al. A novel approach for the definition of the inorganic medium components for micropropagation of yellow passionfruit (Passiflora edulis sims. F. Flavicarpa Deg.). In Vitro Cell.Dev.Biol.-Plant 36, 527–531 (2000). https://doi.org/10.1007/s11627-000-0094-3
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DOI: https://doi.org/10.1007/s11627-000-0094-3