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A Novel Ankyrin Repeat-rich Gene in Potato, Star, Involved in Response to Late Blight

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Abstract

The Solanum tuberosum ankyrin repeat gene (Star) is a novel gene from potato leaves challenged by Phytophthora infestans, a pathogen causing late blight disease. The gene was isolated, based on the reported expressed sequence tag, by the rapid amplification of cDNA ends. Star contains a maximum open reading frame of 1542 bp encoding a peptide with 514 amino acids, and it encodes a RING finger ankyrin repeat protein, a putative E3 ubiquitin ligase. To the authors’ knowledge, it is the first RING finger ankyrin repeat gene isolated from the potato. The gene is highly expressed in roots, stems, and flowers at the transcript level. Star mRNA was strongly expressed from 24 to 72 h in potato leaves inoculated with P. infestans. The results suggested that Star may be involved in the development of organs and may play a role in late blight resistance.

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Acknowledgments

We thank the International Potato Center (CIP) for providing the potato clone 386209.10 with quantitative resistance to late blight. This study was supported by the National 863 Project of China (grant no. 2006AA10Z149).

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Authors and Affiliations

  1. Key Laboratory of Horticulture Plant Biology, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan, 430070, China

    Tian Wu, Zhendong Tian, Jun Liu, Chunguang Yao & Conghua Xie

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  1. Tian Wu
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  2. Zhendong Tian
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  3. Jun Liu
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  4. Chunguang Yao
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  5. Conghua Xie
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Correspondence to Conghua Xie.

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Wu, T., Tian, Z., Liu, J. et al. A Novel Ankyrin Repeat-rich Gene in Potato, Star, Involved in Response to Late Blight. Biochem Genet 47, 439–450 (2009). https://doi.org/10.1007/s10528-009-9238-2

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  • DOI: https://doi.org/10.1007/s10528-009-9238-2

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