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GnRH pulse frequency differentially regulates steroidogenic factor 1 (SF1), dosage-sensitive sex reversal-AHC critical region on the X chromosome gene 1 (DAX1), and serum response factor (SRF): potential mechanism for GnRH pulse frequency regulation of LH beta transcription in the rat

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Abstract

The issue of how rapid frequency GnRH pulses selectively stimulate LH transcription is not fully understood. The rat LHβ promoter contains two GnRH-responsive regions: the proximal region has binding elements for SF1, and the distal site contains a CArG box, which binds SRF. This study determined whether GnRH stimulates pituitary SF1, DAX1 (an endogenous SF1 inhibitor), and SRF transcription in vivo, and whether regulation is frequency dependent. Male rats were pulsed with 25 ng GnRH i.v. every 30 min or every 240 min for 1–24 h, and primary transcripts (PTs) and mRNAs were measured by real time PCR. Fast frequency GnRH pulses (every 30 min) increased SF1 PT (threefold) within 1 h, and then declined after 6 h. SF1 mRNA also increased within 1 h and remained elevated through 24 h. Fast frequency GnRH also stimulated a transient increase in DAX1 PT (twofold after 1 h) and mRNA (1.7-fold after 6 h), while SRF mRNA rose briefly at 1 h. Slow frequency pulses did not affect gene expression of SF1, DAX1, or SRF. These findings support a mechanistic link between SF1 in the frequency regulation of LHβ transcription by pulsatile GnRH.

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Acknowledgment

This research was supported by USPHS Grant HD-33039 (to JCM) and NICHD/NIH through a cooperative agreement (U54-HD28934, Ligand Assay and Analysis Core) as part of the Specialized Cooperative Centers Program in Reproductive Research (JCM, DJH).

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  1. University of Michigan, Medical Sciences Building II, Ann Arbor, MI, 48109, USA

    Laura L. Burger

  2. University of Virginia Health Sciences Center, Aurbach Medical Research Building, P.O. Box 801412, Charlottesville, VA, 22908, USA

    Daniel J. Haisenleder & John C. Marshall

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  1. Laura L. Burger
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Correspondence to Daniel J. Haisenleder.

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Burger, L.L., Haisenleder, D.J. & Marshall, J.C. GnRH pulse frequency differentially regulates steroidogenic factor 1 (SF1), dosage-sensitive sex reversal-AHC critical region on the X chromosome gene 1 (DAX1), and serum response factor (SRF): potential mechanism for GnRH pulse frequency regulation of LH beta transcription in the rat. Endocr 39, 212–219 (2011). https://doi.org/10.1007/s12020-011-9440-y

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