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Analysis of QTL for seed oil content in Brassica napus by association mapping and QTL mapping

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Abstract

Increasing seed oil content is one of the most important breeding targets for rapeseed (Brassica napus). In this study, we combined quantitative trait loci (QTL) mapping and marker-trait association analysis to dissect the genetic basis of seed oil content in rapeseed. A doubled haploid (DH) population with 261 lines was grown in two highly contrasting macro-environments, Germany with winter ecotype environment and China with semi-winter ecotype environment, to explore the effect of environment effect of on seed oil content. Notable macro-environment effect was found for seed oil content. 19 QTL for seed oil content were identified across the two macro-environments. For association analysis, a total of 142 rapeseed breeding lines with diverse oil contents were grow in China macro-environment. We identified 23 simple sequence repeat (SSR) markers that were significantly associated with the seed oil content. Comparative analysis revealed that five QTL identified in the DH population, located on chromosomes A03, A09, A10 and C09, were co-localized with 11 significantly associated SSR markers that were identified from the association mapping population. Of which, the QTL on chromosome A10 was found to be homeologous with the QTL on chromosome C09 by aligning QTL confidence intervals with the reference genomes B. napus. Those QTL associated with specific macro-environments provides valuable insight into the genetic regulation of seed oil content and will facilitate marker-assisted breeding of B. napus.

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Acknowledgements

This study was supported financially by Project of Young Researcher Cultivation of Zhejiang Academy of Agricultural Sciences; Project of Hybrid Breeding of High Oil Content in Rapeseed (2011R50026-2); The Key Project of Novel Variety Breeding of Zhejiang Province (2012C12902-1).

Authors’ contributions

HY, WQ and YF designed research; YF, DZ and HD performed research, YF, YZ, BL, and SH analyzed data; YF, MG, JL and FM wrote the paper.

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Authors and Affiliations

  1. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, China

    Ying Fu, Dongqing Zhang, Yaofeng Zhang, Baogang Lin, Shuijin Hua, Houdong Ding & Huasheng Yu

  2. College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China

    Jiana Li & Wei Qian

  3. Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Level 3 South, Queensland Biosciences Precinct, St Lucia, QLD, Australia

    Madeleine Gleeson

  4. Norddeutsche Pflanzenzucht Hans-Georg Lembke KG, Hohenlieth, 24363, Germany

    Martin Frauen

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  1. Ying Fu
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  2. Dongqing Zhang
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  3. Madeleine Gleeson
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  4. Yaofeng Zhang
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  5. Baogang Lin
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  10. Wei Qian
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Correspondence to Wei Qian or Huasheng Yu.

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Fu, Y., Zhang, D., Gleeson, M. et al. Analysis of QTL for seed oil content in Brassica napus by association mapping and QTL mapping. Euphytica 213, 17 (2017). https://doi.org/10.1007/s10681-016-1817-9

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