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Superoxide dismutase 1 modulates expression of transferrin receptor

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Abstract

Copper–zinc superoxide dismutase (SOD1) plays a protective role against the toxicity of superoxide, and studies in Saccharomyces cerevisiae and in Drosophila have suggested an additional role for SOD1 in iron metabolism. We have studied the effect of the modulation of SOD1 levels on iron metabolism in a cultured human glial cell line and in a mouse motoneuronal cell line. We observed that levels of the transferrin receptor and the iron regulatory protein 1 were modulated in response to altered intracellular levels of superoxide dismutase activity, carried either by wild-type SOD1 or by an SOD-active amyotrophic lateral sclerosis (ALS) mutant enzyme, G93A-SOD1, but not by a superoxide dismutase inactive ALS mutant, H46R-SOD1. Ferritin expression was also increased by wild-type SOD1 overexpression, but not by mutant SOD1s. We propose that changes in superoxide levels due to alteration of SOD1 activity affect iron metabolism in glial and neuronal cells from higher eukaryotes and that this may be relevant to diseases of the nervous system.

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Abbreviations

ALS:

Amyotrophic lateral sclerosis

FALS:

Familial amyotrophic lateral sclerosis

HEPES:

N′-(2-Hydroxyethyl)piperazine-N′-ethanesulfonic acid

IRE:

Iron responsive element

IRP:

Iron regulatory protein

mRNA:

Messenger RNA

mRNP:

Messenger ribonucleoprotein

ROS:

Reactive oxygen species

SDS:

Sodium dodecyl sulfate

SOD:

Superoxide dismutase

SOD1:

Cu,Zn superoxide dismutase

SOD2:

Exclusively mitochondrial, Mn-containing enzyme

SOD3:

Extracellular Cu,Zn protein

TfR:

Transferrin receptor

Tris:

Tris(hydroxymethyl)aminomethane

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Acknowledgements

We are grateful to Luisa Rossi and Laura Scarino for helpful discussion, to Cecile Bouton and Cedric Fosset for supplying the IRE plasmid pSPT-fer and for help in establishing the band-shift assay protocol and to Neil R. Cashman for the gift of the original NSC34 cell line. We are indebted to Evamaria Görz for invaluable technical support. This work was supported by Telethon (GGP030066 to M.T.C.), by Deutsche Forschungsgemeinschaft (grant DA 559/2-1 to R.D.), by GIF (German–Israeli Foundation for Scientific Research and Development; grant no. 2066-1212.2/2002 to R.D.), Min. Salute (RF Malattie Neurodegenerative to M.T.C.), and a National Institutes of Health grant DK46828 to J.S.V.

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Authors and Affiliations

  1. Department of Neurology, University of Ulm, Ulm, Germany

    Ruth Danzeisen, Ulrich Bederke, Malte Frenzel, Lea Huber & Albert Ludolph

  2. Fondazione Santa Lucia IRCCS, Rome, Italy

    Tilmann Achsel, Mauro Cozzolino, Claudia Crosio, Alberto Ferri, Monica Nencini & Maria Teresa Carrì

  3. DiFBC, University of Sassari, Sassari, Italy

    Claudia Crosio

  4. Department of Psychobiology and Psychopharmacology, CNR Institute for Neuroscience, Rome, Italy

    Alberto Ferri

  5. Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA, 90095-1569, USA

    Edith Butler Gralla & Joan Selverstone Valentine

  6. Department of Biology, University of Rome “Tor Vergata”, Via della Ricerca Scientifica, 00133, Rome, Italy

    Giuseppe Rotilio & Maria Teresa Carrì

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  1. Ruth Danzeisen
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  2. Tilmann Achsel
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  3. Ulrich Bederke
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  10. Albert Ludolph
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  14. Maria Teresa Carrì
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Corresponding author

Correspondence to Maria Teresa Carrì.

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Danzeisen, R., Achsel, T., Bederke, U. et al. Superoxide dismutase 1 modulates expression of transferrin receptor. J Biol Inorg Chem 11, 489–498 (2006). https://doi.org/10.1007/s00775-006-0099-4

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  • DOI: https://doi.org/10.1007/s00775-006-0099-4

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