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Evidence that heteronuclear proteins interact with the XIST RNA in vitro

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Somatic Cell and Molecular Genetics

Abstract

The process of X chromosome inactivation results in the transcriptional silencing of one of the two X chromosomes in mammalian females. A large heterogeneous nuclear RNA that is expressed exclusively from the inactive X chromosome (XIST—X Inactive Specific Transcripts) has been implicated in the inactivation process. The XIST RNA colocalizes with the inactive X chromosome and therefore proteins that interact with the XIST RNA may be involved in the inactivation of the X chromsome. In order to identify such proteins we have used anin vitro UV light cross-linking technique to detect nuclear proteins associating with sections of the XIST RNA. The strongest interaction detected by this technique was between a pair of approximately 40 kDa proteins and a 5′ region of the XIST RNA which contains a series of well-conserved tandem repeats. Immunoprecipitation suggested that these proteins may be the heteronuclear proteins hnRNPC1/C2.

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  1. Department of Medical Genetics, University of British Columbia, V6T 1Z3, Vancouver, British Columbia

    Carolyn J. Brown & Sarah E. L. Baldry

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  1. Carolyn J. Brown
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Brown, C.J., Baldry, S.E.L. Evidence that heteronuclear proteins interact with the XIST RNA in vitro. Somat Cell Mol Genet 22, 403–417 (1996). https://doi.org/10.1007/BF02369896

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  • DOI: https://doi.org/10.1007/BF02369896

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