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Molecular Cloning and Characterization of a Novel Canine Sulfotransferase

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Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 16))

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Abstract

Sulfonation, as mediated by the cytosolic sulfotransferases (SULTs), represents an important mechanism in vivo for the detoxification/metabolism of drugs and other xenobiotics, as well as endogenous neurotransmitters and hormones. Madin-Darby canine kidney (MDCK) cells have been widely used as a model system for investigating the excretion/detoxification mechanism of kidney. In this study, a cDNA encoding a novel canine SULT was cloned from MDCK cells using reverse transcription-polymerase chain reaction (RT-PCR) technique. Nucleotide sequence revealed that the newly cloned canine SULT cDNA has an open reading frame encompassing 912 nucleotides and encoding a 303-amino acid polypeptide. Analysis of the deduced amino acid sequence showed that the novel canine SULT is highly homologous to human SULT1C4, with 82.8% amino acid identity. Recombinant canine SULT1C4, expressed and purified using the pGEX-4T-1 GST gene fusion system, exhibited strong phenol-sulfonating activities toward 1- or 2-naphthol and o-bromophenol among a variety of xenobiotic compounds tested as substrates. These data indicated that the newly identified canine SULT1C4 may function in kidney for the detoxification of xenobiotic compounds.

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Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research (B), (C) (M.S. and Y.S.), a Grant-in Aid for Encouragement of Young Scientists (Y.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Health and Sciences Research Grants (Toxicogenomics) from the Ministry of Health, Labor and Welfare of Japan (Y.S.) and start-up funds from College of Pharmacy, The University of Toledo (M.C.L.). We also thank Satoshi Miyazaki, Saki Takahashi, Dr Madhyasha Harish Kumar for great help of this study and manuscript.

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Authors and Affiliations

  1. Department of Biochemistry and Applied Biosciences, University of Miyazaki, Miyazaki, 889-2192, Japan

    Katsuhisa Kurogi, Yoichi Sakakibara & Masahito Suiko

  2. Department of Bioscience, School of Agriculture, Tokai University, 869-1404, Aso, Kumamoto, Japan

    Shin Yasuda

  3. Department of Pharmacology, College of Pharmacy, The University of Toledo, 43606, Toledo, OH, USA

    Ming-Cheh Liu

Authors
  1. Katsuhisa Kurogi
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  2. Yoichi Sakakibara
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  3. Shin Yasuda
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  4. Ming-Cheh Liu
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  5. Masahito Suiko
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Corresponding author

Correspondence to Katsuhisa Kurogi .

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Editors and Affiliations

  1. Fac. Engineering, Dept. Chemical Engineering, Kyushu University, Motooka 744, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

  2. Fac. Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Yoshinori Katakura

  3. Fac. Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Akira Ito

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Kurogi, K., Sakakibara, Y., Yasuda, S., Liu, MC., Suiko, M. (2010). Molecular Cloning and Characterization of a Novel Canine Sulfotransferase. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_36

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