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Synthesis of C-Terminal Peptide Thioesters Using Fmoc-Based Solid-Phase Peptide Chemistry

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Peptide Synthesis and Applications

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1047))

Abstract

This chapter provides two protocols for the solid-phase synthesis of peptide thioesters using N α-Fmoc-protected amino acids. The first protocol is based on a so-called safety-catch linker, while the second relies on a backbone amide linker.

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Author information

Authors and Affiliations

  1. IGM, Faculty of Life Sciences, University of Copenhagen, Zealand Pharma, Glostrup, Denmark

    Pernille Tofteng Shelton

  2. Department of Chemistry, Faculty of Sciences, University of Copenhagen, Frederiksberg, Denmark

    Knud J. Jensen

Authors
  1. Pernille Tofteng Shelton
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  2. Knud J. Jensen
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Editor information

Editors and Affiliations

  1. IGM, Faculty of Sciences, University of Copenhagen, Frederiksberg, Denmark

    Knud J. Jensen

  2. IGM, Faculty of Science, University of Copenhagen, Glostrup, Denmark

    Pernille Tofteng Shelton

  3. IGM, Faculty of Life Sciences, University of Copenhagen, Hørsholm, Denmark

    Søren L. Pedersen

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Shelton, P.T., Jensen, K.J. (2013). Synthesis of C-Terminal Peptide Thioesters Using Fmoc-Based Solid-Phase Peptide Chemistry. In: Jensen, K., Tofteng Shelton, P., Pedersen, S. (eds) Peptide Synthesis and Applications. Methods in Molecular Biology, vol 1047. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-544-6_8

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  • DOI: https://doi.org/10.1007/978-1-62703-544-6_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-543-9

  • Online ISBN: 978-1-62703-544-6

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