Abstract
Staphylococcal secreted nuclease contributes to S. aureus virulence by degrading neutrophil extracellular traps (NETs), which allows the bacterium to evade the host immune system and has also been shown to promote biofilm dispersal. In this chapter, two methods for detecting nuclease activity are described, both of which have increased sensitivity compared to the traditional nuclease agar method.
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Acknowledgments
This work was supported in part by grant AI128376 from the National Institute of Allergy and Infectious Diseases.
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Wiemels, R.E., Keogh, R.A., Carroll, R.K. (2021). Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants. In: Rice, K.C. (eds) Staphylococcus aureus. Methods in Molecular Biology, vol 2341. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1550-8_3
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DOI: https://doi.org/10.1007/978-1-0716-1550-8_3
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