Skip to main content
SpringerLink
Log in

Staphylococcal nuclease reviewed: A prototypic study in contemporary enzymology

IV. The nuclease as a model for protein folding

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

This is the last in a series of four articles in which the chemical, enzymological and crystallographic work on Ribonucleate (deoxyribonucleate)-3′-nucleotidohydrolase, EC 3.1.4.4 (staphylococcal nuclease, micrococcal nuclease) will be reviewed and correlated. This article discusses the use of the nuclease as a model system for the study of the mechanisms and energetics of the folding-unfolding reaction in proteins and for the study of the interrelationships between amino acid sequence and three-dimensional structure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tucker, P. W., Cotton, F. A. and Hazen, E. E., Jr., 1978. Mol. Cell. Biochem. 22, 67

    Google Scholar 

  2. Tucker, P. W., Cotton, F. A. and Hazen, E. E., Jr., ibid, vol 23, 3

    Google Scholar 

  3. Tucker, P. W., Cotton, F. A. and Hazen, E. E., Jr., ibid, 23, 67

    Google Scholar 

  4. Anfinsen, C. B., 1973. Science, 181, 223.

    Google Scholar 

  5. Anfinsen, C. B. and Sheraga, H. A., 1975. Advan. Protein Chem. 29, 205.

    Google Scholar 

  6. Schechter, A. N., Chen, R. F., and Anfinsen, C. B., 1970. Science, 167, 886.

    Google Scholar 

  7. Epstein, A. F., Schechter, A. N., Chen, R. F., and Anfinsen, C. B., 1971. J. Mol. Biol. 60, 449.

    Google Scholar 

  8. Hammes, G. G., and Roberts, P. B., 1969. J. Amer. Chem. Soc. 91, 1812.

    Google Scholar 

  9. Cuatrecasas, P., Taniuchi, H., and Anfinsen, C. B., 1969. Brookhaven Symp. Biol. 21, 172.

    Google Scholar 

  10. Taniuchi, H., and Anfinsen, C. B., 1966. J. Biol. Chem 241, 4366.

    Google Scholar 

  11. Tucker, P. W., 1975. Ph.D. Dissertation, Texas A&M University.

  12. Markley, J. L., 1969. Ph.D. Thesis, Harvard University.

  13. Taniuchi, H., and Bohnert, J. L., 1975, J. Biol. Chem. 250, 2388.

    Google Scholar 

  14. Epstein, A. F., Schechter, A. N. and Cohen, J. S., 1972. Proc. Nat. Acad. Sci. USA 69, 3790.

    Google Scholar 

  15. Anfinsen, C. B., Schechter, A. N. and Taniuchi, H., 1972. Cold Spring Harbor Symp. Quant. Biol. 36, 249.

    Google Scholar 

  16. Markley, J. L., and Jardetzky, O., 1970. J. Mol. Biol. 50,223.

    Google Scholar 

  17. Markely, J. L., Williams, M. N., and Jardetzky, O., 1970. Proc. Nat. Acad. Sci. USA 65, 395.

    Google Scholar 

  18. Arata, Y., Khalifah, R., and Jardetzky, O., 1973. Ann. N.Y. Acad. Sci. 222, 230.

    Google Scholar 

  19. Jardetzky, O., Markley, J. L., Williams, M. N., Thielmann, H., and Arata, Y. Cold Spring Harbor Symp. Quant. Biol. 36, 257.

  20. Cohen, J. S., Shraga, R. I., McNell, M., and Schechter, A. N., 1970. Nature, 228, 642.

    Google Scholar 

  21. Sulkowski, E., and Laskowski, M., Sr., 1968. J. Biol. Chem. 243, 4917.

    Google Scholar 

  22. Arnone, A., Bier, C. J., Cotton, F. A., Hazen, E. E., Jr., Richardson, N. C., Richardson, J. S., Day, V. W., and Yonath, A., 1971. J. Biol. Chem. 246, 2302.

    Google Scholar 

  23. Taniuchi, H., Anfinsen, C. B., and Sodja, A., 1967. Proc. Nat Acad. Sci., USA 58, 1235.

    Google Scholar 

  24. Anfinsen, C. B., 1968. Dev. Biol. Suppl. 2, 1.

    Google Scholar 

  25. Taniuchi, H. and Anfinsen, C. B., 1971. J. Biol. Chem. 246, 2291.

    Google Scholar 

  26. Taniuchi, H., and Anfinsen, C. B., 1968. J. Biol. Chem. 243, 4778.

    Google Scholar 

  27. Taniuchi, H., Moravek, L., and Anfinsen, C. B., 1969 J. Biol. Chem. 244, 4600.

    Google Scholar 

  28. Taniuchi, H. and Anfinsen, C. B., 1969. J. Biol. Chem. 244, 3864.

    Google Scholar 

  29. Chaiken, I. M., 1971. J. Biol. Chem., 246, 2948.

    Google Scholar 

  30. Chaiken, I. M., and Anfinsen, C. B., 1970. J. Biol. Chem. 245, 4718.

    Google Scholar 

  31. Chaiken, I. M., 1972. J. Biol. Chem. 247, 1999.

    Google Scholar 

  32. Ontjes, D. A., and Anfinsen, C. B., 1969. J. Biol. Chem. 244, 6316.

    Google Scholar 

  33. Parikh, I., Corley, I., and Anfinsen, C. B., 1971. J. Biol. Chem. 246, 7392.

    Google Scholar 

  34. Cotton, F. A. and Hazen, E. E., Jr., 1971. The Enzymes. (Boyer, P. D., ed:) Vol. 3, erd Ed., p. 153, Academic Press, New York.

  35. Cotton, F. A., Bier, C. J., Day, V. W., Hazen, E. E., Jr., and Larsen, S., 1971. Cold Spring Harbor Symp. Quant. Biol. 34, 243.

    Google Scholar 

  36. Taniuchi, H., 1970. Fed. Proc. 29, 335.

    Google Scholar 

  37. Andria, G., Taniuchi, H., and Cone, J. L., 1971. J. Biol. Chem. 246, 7421.

    Google Scholar 

  38. Taniuchi, H., 1973. J. Biol. Chem. 248, 5164.

    Google Scholar 

  39. Taniuchi, H., Davies, D. R., and Anfinsen, C. B., 1973. J. Biol. Chem. 247, 3362.

    Google Scholar 

  40. Light, A., Taniuchi, H., and Chen, R. F., 1974. J. Biol. Chem. 249, 2285.

    Google Scholar 

  41. Taniuchi, H., Parker, D. S. and Bohnert, J. L., 1977. J. Biol. Chem. 252, 125.

    Google Scholar 

  42. Sachs, D. H., Schechter, A. N., Eastlake, A., and Anfinsen, C. B., 1972. J. Immunol. 109, 1300.

    Google Scholar 

  43. Sach, D. H., Schechter, A. N., Eastlake, A., and Anfinsen, C. B., 1974. Nature 251, 242.

    Google Scholar 

  44. Sach, D. H., Schechter, A. N., Eastlake, A., and Anfinsen, C. B., 1972. Biochemistry 11, 4268.

    Google Scholar 

  45. Eastlake, A., Sachs, D. H., Schechter, A. N., and Anfinsen, C. B., 1974. Biochemistry 13, 1567.

    Google Scholar 

  46. Omenn, G. S., Ontjes, D. H., and Anfinsen, C. B., 1970. Biochemistry, 9, 304.

    Google Scholar 

  47. Omenn, G. S., Ontjes, D. A., and Anfinsen, C. B. ibid p. 313.

    Google Scholar 

  48. Furie, B., Schechter, A. N., Sachs, D. H., and Anfinsen, C. B., 1974. 13, 1561.

  49. Sachs, D. H., Schechter, A. N., Eastlake, A., and Anfinsen, C. B., 1972, Proc. Nat. Acad. Sci. USA 69, 3790.

    Google Scholar 

  50. Furie, B., Schechter, A. N., Sachs, D. H., and Anfinsen, C. B., 1975. 92, 497.

  51. Tanford, C., 1970. Advan. Protein Chem. 24, 1.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Genetics, University of Wisconsin-Madison, 53706, Madison, Wisconsin

    Philip W. Tucker

  2. Department of Chemistry, Texas A&M University, Area code 713, 845-5345, 77843, College Station, Texas

    Edward E. Hazen Jr. & F. Albert Cotton

Authors
  1. Philip W. Tucker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Edward E. Hazen Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Albert Cotton
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This article is the last in a series of four devoted to the staphylococcal nuclease. The previous articles have discussed its isolation and enzymology, the chemical and enzymological studies of its active site and the high-resolution crystallographic work. The third article also put forth a mechanism for the enzymatic reaction based on the nuclease's known chemical and structural properties. Work from this laboratory has been supported by grants from the National Institute of General Medical Sciences, N.I.H. and the Robert A. Welch Foundation to F. A. Cotton and from the Welch Foundation to E. E. Hazen, Jr.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tucker, P.W., Hazen, E.E. & Cotton, F.A. Staphylococcal nuclease reviewed: A prototypic study in contemporary enzymology. Mol Cell Biochem 23, 131–141 (1979). https://doi.org/10.1007/BF00219452

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00219452

Index Entries

Search

Navigation