Indian Journal of Pharmacy and Pharmacology

Print ISSN: 2393-9079

Online ISSN: 2393-9087

CODEN : IJPPTK

Indian Journal of Pharmacy and Pharmacology (IJPP) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award programs. With more...

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113-124


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Veeresh P Veerapur*, Nagarjun S, Vivek Chandramohan, Vijayakumar S


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Chromium (D-Phenylalanine)3 supplementation attenuates acetic acid induced ulcerative colitis in rats: In silico docking and dynamic studies using NF-kB


Original Article

Author Details : Veeresh P Veerapur*, Nagarjun S, Vivek Chandramohan, Vijayakumar S

Volume : 7, Issue : 2, Year : 2020

Article Page : 113-124

https://doi.org/10.18231/j.ijpp.2020.020



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Abstract

Background: chromium-D-phenylalanine [Cr(D-phe)3] is known to be anti-diabetic, anti-inflammatory
and antioxidant complex. Our preliminary work reveals the beneficial effect of Cr(D-phe)3 in indomethacininduced
enterocolitis.
Aim: The present work was intended to explore the effect of Cr(D-phe)3 in acetic acid-induced ulcerative
colitis in rats. Further, molecular docking simulation experiments were performed.
Methodology: Colitis was induced through intra-rectal instillation of acetic acid (3% v/v) and the
effectiveness of Cr(D-phe)3 (30, 60 and 90 mg/kg) and sulphasalazine was measured using clinical,
macroscopic, biochemical, contractility and histopathological studies. In addition, drug likeliness,
molecular docking and dynamic studies of Cr(D-phe)3 and sulphasalazine with NF-kappa B (1NFK) were
carried out.
Results: Pretreatment of different doses of Cr(D-phe)3 showed significant reduction (P<0> in clinical, macroscopic score, oxidative stress and elevated biochemical parameters. Protective nature of
Cr(D-phe)3was further confirmed by histopathological examination and colonic contractility studies. In
silico studies reveals that Cr(D-phe)3 exhibited better docking score (-14.121) compared to sulphasalazine
(-5.654). Dug likeliness studies showed that Cr(D-phe)3 passes lipinski’s rule and exhibited better
bioavailability properties with negligible hepatotoxicity compared to standard. Molecular dynamic studies
reveal that Cr(D-phe)3 showed better stability compared to standard compound, while interacting with
1NFK for 10 ns.
Conclusion: The observed beneficial activity of Cr(D-phe)3 could be due to its anti-oxidative and antiinflammation
by preventing NF-kB activity.

Keywords: Inflammatory Bowel Disease, Cr(D-phe)3, Colonic contractility, Oxidative stress, Drug likeliness, 1NFK.


How to cite : Veerapur V P , Nagarjun S, Chandramohan V , Vijayakumar S, Chromium (D-Phenylalanine)3 supplementation attenuates acetic acid induced ulcerative colitis in rats: In silico docking and dynamic studies using NF-kB. Indian J Pharm Pharmacol 2020;7(2):113-124

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