International Journal of Clinical Biochemistry and Research

Print ISSN: 2394-6369

Online ISSN: 2394-6377

CODEN : IJCBK6

International Journal of Clinical Biochemistry and Research (IJCBR) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award more...

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Original Article


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430-436


Authors Details

Mohammad Najmuddin Khan*, Seema Goel


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Binding study of different drugs with serum albumins


Original Article

Author Details : Mohammad Najmuddin Khan*, Seema Goel

Volume : 7, Issue : 4, Year : 2020

Article Page : 430-436

https://doi.org/10.18231/j.ijcbr.2020.091



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Abstract

Objective: Besides bilirubin and fatty acids are more important among the physiological ligands transported by albumin, drugs constitute the major class of exogenous ligands transported by albumin.
Considering the total number of ligands bound to albumin, it is inconceivable to think of the same number of ligand binding sites on albumin molecule, so it seems that same binding sites are shared by several compounds. Thus it is possible that some ligands may be displaced from albumin by other ligands. Various drugs are well known displacer of bilirubin from albumin.
Materials and Methods: In the present communication the binding of three drugs namely, Indomethacin, chlorpromazine and oxyphenbutazone to serum albumins (BSA and SSA) under different conditions of pH and ionic strength have been studied by fluorescence quenching technique in order to ascertain the role of various non – covalent interactions.
Results and Discussion: The results suggest that in case of indomethacin, a decrease in binding was observed on increasing the ionic strength, indicating the involvement of electrostatic interactions whereas in case of chlorpromazine and oxyphenbutazone, significant increase in binding was noticed on increasing the ionic strength was suggestive of the involvement of hydrophobic interactions. The extent of binding of these drugs was more pronounced in BSA compared to SSA.

Keywords: Sheep serum albumin, Bovine serum albumin, Human serum albumin, Bilirubin binding, Ionic strength, Fluorescence quench, Indomethacin, Chlorpromazine, Oxyphenbutazone.


How to cite : Khan M N , Goel S , Binding study of different drugs with serum albumins. Int J Clin Biochem Res 2020;7(4):430-436

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