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Estimation of eugenol in different tulsi ayurvedic formulations by RP-HPLC method

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Original Article

Author Details : Sreenivasa Charan Archakam*, Keerthisikha Palur, Mohan Krishna Yerragunta, Bhaskar Kuruba Pujari

Volume : 7, Issue : 1, Year : 2022

Article Page : 51-53

https://doi.org/10.18231/j.ijcaap.2022.009

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Abstract

Aim: The aim of the present is to develop simple, accurate and precise RP-HPLC method for the quantification of Eugenol content in various tulsi based ayurvedic formulations.
Materials and Methods: HPLC was carried out by reverse –phase technique on a C18 column with a mobile phase composed of Acetonitrile: Methanol in the proportion of 50:50 v/v, at a flow rate of 1.0 mL /min. The detection was carried at 281 nm by UV- Visible detector. The linearity was established in the concentration range of 10-50 mcg/mL with an R value of >0.999. Method and system precision was established with an RSD of < 2> Results: The method was found to be specific, precise, accurate and robust. The proposed method can be used for the reliable quantification of Eugenol.

Keywords: Eugenol, Tulsi, RP­HPLC, Accuracy, Precision, Ayurvedic formulations

How to cite : Archakam S C, Palur K, Yerragunta M K, Pujari B K, Estimation of eugenol in different tulsi ayurvedic formulations by RP-HPLC method. IP Int J Compr Adv Pharmacol 2022;7(1):51-53

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