In the course of isolating soil microorganisms four strains of actinomycetes were isolated. Based on the morphological studies these organisms were found to belong to a new genus in the family Actinoplanaceae. These organisms produced abundant aerial mycelia as Streptomycetaceae, and produced a clubshaped sporangium and a motile zoospore. With these characters it was designated as Kitasatoa gen. nov., belonging to the family Actinoplanaceae. Among four strains, Kitasatoa purpurea is the type genus. The three other strains are: K. kauaiensis, K. nagasakiensis and K. diplospora.