Czech J. Food Sci., 2013, 31(5):514-519 | DOI: 10.17221/507/2012-CJFS

Rapid immunoassays for detection of anabolic nortestosterone in dietary supplementsOriginal Paper

Barbora HOLUBOVÁ1, Sandra GÖSELOVÁ1, Ludmila ŠEVČÍKOVÁ2, Miroslav VLACH1, Martina BLAŽKOVÁ1, Oldřich LAPČÍK2, Ladislav FUKAL1
1 Department of Biochemistry and Microbiology and
2 Department of Chemistry of Natural Compounds, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic

An enzyme immunoassay (ELISA) and an immunochromatographic strip were designed for a rapid detection of nortestosterone in dietary supplements. Two polyclonal antibodies and two types of nortestosterone-protein coating conjugates were tested to develop the most appropriate method. Under optimal experimental conditions, the most sensitive ELISA achieved the IC50 and the limit of detection values of 6.41 and 0.09 ng/ml, respectively. The assay specificity was tested measuring cross-reactivity of several steroids. The interference with the assay was negligible (< 0.1%), except for cross-reactivity with another frequently abused steroid testosterone (23%). The optimised gold particle-based immunochromatographic strip provided in semi-quantitative test a visual detection limit of 1 ng/ml. None of these methods showed the interference using a filtrate of the suspension of non-contaminated sample. After the validation for particular matrices, the ELISA and the strip test could be useful tools for a rapid analysis of nortestosterone in crude extracts of dietary supplements.

Keywords: 19-nortestosterone; ELISA; colloidal gold immunoassay; strip test

Published: October 31, 2013  Show citation

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HOLUBOVÁ B, GÖSELOVÁ S, ŠEVČÍKOVÁ L, VLACH M, BLAŽKOVÁ M, LAPČÍK O, FUKAL L. Rapid immunoassays for detection of anabolic nortestosterone in dietary supplements. Czech J. Food Sci.. 2013;31(5):514-519. doi: 10.17221/507/2012-CJFS.
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