Hörterer, Christina; Lannig, Gisela; Buck, Bela Hieronymus (2024): Lipid content of liver and muscle tissue of juvenile turbot (Scophthalmus maximus) fed with emerging protein sources [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960775
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Abstract:
This study examined the growth response of juvenile turbot (Scophthalmus maximus) to diets with graded fishmeal (FM) replacement with plant, animal, and emerging protein sources (PLANT, PAP, and MIX) in comparison to a commercial-like diet (CTRL). The feeding experiment was carried out from April to July 2019 in the Centre for Aquaculture Research (ZAF) at the Alfred Wegener Institute for Polar and Marine research in Bremerhaven, Germany. The juvenile turbot (Scophthalmus maximus) were purchased from France Turbot (L'Épine, France) and acclimated to the recirculating aquaculture system (RAS) for 2 weeks prior to starting the 16 weeks experimental trial. To elucidate the effects of the protein sources and the level of FM replacement on the nutritional status of the fish lipid content of liver and muscle tissue were determined at the end of the experiment (t4). Muscle and liver samples (3 individual fish per tank; 15 fish per diet in total) were grinded under liquid nitrogen. Due to small volume, the individual samples of liver and muscle were pooled (n=5 tanks per diet) for the crude lipid content. Following the method of Folch et al. (1957) and Postel et al. (2000), the lipids in the muscle and liver tissue were extracted with 2:1 dichloromethanemethanol (v/v) and an aqueous solution of 0.88% potassium chloride (KCl) (w:v). Crude lipid content was determined gravimetrically to the nearest 0.001 g and calculated as the percentage of lipids of tissue wet weight.
Related to:
Hörterer, Christina; Petereit, Jessica; Lannig, Gisela; Bock, Christian; Buck, Bela Hieronymus (2023): 1H-NMR-Based Metabolic Profiling in Muscle and Liver Tissue of Juvenile Turbot (Scophthalmus maximus) Fed with Plant and Animal Protein Sources. Metabolites, 13(5), 612, https://doi.org/10.3390/metabo13050612
Hörterer, Christina; Petereit, Jessica; Lannig, Gisela; Johansen, Johan; Pereira, Gabriella V; Conceição, Luis E C; Pastres, Roberto; Buck, Bela Hieronymus (2022): Sustainable fish feeds: potential of emerging protein sources in diets for juvenile turbot (Scophthalmus maximus) in RAS. Aquaculture International, 30, 1481-1504, https://doi.org/10.1007/s10499-022-00859-x
References:
Folch, Jordi; Lees, M; Sloane Stanley, G H (1957): A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry, 226(1), 497-509, https://doi.org/10.1016/S0021-9258(18)64849-5
Postel, Lutz; Fock, Heino O; Hagen, Wilhelm (2000): Biomass and abundance. In: Harris, R.; Wiebe, P; et al. (eds.), ICES Zooplankton Methodology Manual, San Diego: Acad. Press, 83-192, https://doi.org/10.1016/B978-012327645-2/50005-0
Funding:
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Hörterer, Christina | GAIN (project)_SM1 (Species; Experiment 1) | ||
| 2 | Type of study | Study type | Hörterer, Christina | |||
| 3 | Species, unique identification | Species UID | Hörterer, Christina | |||
| 4 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Hörterer, Christina | |||
| 5 | Species, unique identification (URI) | Species UID (URI) | Hörterer, Christina | |||
| 6 | Location | Location | Hörterer, Christina | |||
| 7 | Method comment | Method comm | Hörterer, Christina | System: Centre for Aquaculture Research (ZAF), Recirculating Aquaculture System (KL) #1 | ||
| 8 | Sampling date/time, experiment | Date/time sampling exp | Hörterer, Christina | Date fish was sampled from tank | ||
| 9 | Time point, descriptive | Time point | Hörterer, Christina | final sampling at end of experiment | ||
| 10 | Tank number | Tank No | Hörterer, Christina | Fibre glas tanks, 0.7 m3, total number 36 | ||
| 11 | Identification | ID | Hörterer, Christina | Fish#: Individual Fish sampled | ||
| 12 | Sample ID | Sample ID | Hörterer, Christina | sampling point_Tank | ||
| 13 | Treatment | Treat | Hörterer, Christina | Formulation concept: CTRL - commercial-like diet; PLANT - plant protein sources, PAP - animal protein source, MIX - emerging protein source | ||
| 14 | Treatment | Treat | Hörterer, Christina | FMR - Fishmeal replacement level (0; 20; 40) | ||
| 15 | Scophthalmus maximus, liver lipid, per dry mass | S. maximus liver lipid dm | mg/g | Hörterer, Christina | Laboratory balance, Sartorius, Secura 5102-1S | concentration in the freeze-dried tissue determined after Folch et al. (1957) and Postel et al. (2000) |
| 16 | Scophthalmus maximus, muscle lipid, per dry mass | S. maximus muscle lipid dm | mg/g | Hörterer, Christina | Laboratory balance, Sartorius, Secura 5102-1S | concentration in the freeze-dried tissue determined after Folch et al. (1957) and Postel et al. (2000) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
280 data points