How stereochemistry of lipid components can affect lipid organization and the route of liposome internalization into cells

Stefano Borocci; Giuseppina Bozzuto; Cecilia Bombelli; Francesca Ceccacci; Giuseppe Formisano; Annarita Stringaro; Agnese Molinari; Giovanna Mancini

doi:10.1039/D1NR02175C

How stereochemistry of lipid components can affect lipid organization and the route of liposome internalization into cells†

Stefano Borocci,

*^ab Giuseppina Bozzuto,

^c Cecilia Bombelli,

^d Francesca Ceccacci,

^d Giuseppe Formisano,^c Annarita Stringaro,

^c Agnese Molinari

*^c and Giovanna Mancini

*^b

Author affiliations

* Corresponding authors

^a Dipartimento per la Innovazione nei sistemi Biologici, Agroalimentari e Forestali (DIBAF), Università degli Studi della Tuscia, L.go dell'Università, s.n.c., 01100 Viterbo, Italy
E-mail: borocci@unitus.it

^b CNR, Istituto per i Sistemi Biologici, Area della Ricerca di Roma 1, SP35d 9, 00010 Montelibretti, Roma, Italy
E-mail: giovanna.mancini@cnr.it

^c Centro Nazionale per la Ricerca e la Valutazione preclinica e clinica dei Farmaci, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy
E-mail: agnese.molinari@iss.it

^d CNR, Istituto per i Sistemi Biologici, Sede Secondaria di Roma-Meccanismi di Reazione c/o Dipartimento di Chimica Università degli Studi di Roma “Sapienza”, P.le A. Moro 5, 00185 Roma, Italy

Abstract

Though liposome-based drugs are in clinical use, the mechanism of cell internalization of liposomes is yet an object of controversy. The present experimental investigation, carried out on human glioblastoma cells, indicated different internalization routes for two diastereomeric liposomes. Molecular dynamics simulations of the lipid bilayers of the two formulations indicated that the different stereochemistry of a lipid component controls some parameters such as area per lipid molecule and fluidity of lipid membranes, surface potential and water organization at the lipid/water interface, all of which affect the interaction with biomolecules and cell components.

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DOI: https://doi.org/10.1039/D1NR02175C
Article type: Paper
Submitted: 07 Apr 2021
Accepted: 09 May 2021
First published: 02 Jul 2021
This article is Open Access

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Nanoscale, 2021,13, 11976-11993

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How stereochemistry of lipid components can affect lipid organization and the route of liposome internalization into cells

S. Borocci, G. Bozzuto, C. Bombelli, F. Ceccacci, G. Formisano, A. Stringaro, A. Molinari and G. Mancini, Nanoscale, 2021, 13, 11976 DOI: 10.1039/D1NR02175C

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How stereochemistry of lipid components can affect lipid organization and the route of liposome internalization into cells†

Abstract

Supplementary files

Article information

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How stereochemistry of lipid components can affect lipid organization and the route of liposome internalization into cells

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