Elsevier

Cellular Immunology

Volume 298, Issues 1–2, November–December 2015, Pages 104-114
Cellular Immunology

Research paper
Microparticulate β-glucan vaccine conjugates phagocytized by dendritic cells activate both naïve CD4 and CD8 T cells in vitro

https://doi.org/10.1016/j.cellimm.2015.10.007Get rights and content
Under a Creative Commons license
open access

Highlights

  • Yeast microparticulate β-glucan conjugated with ovalbumin (MG:OVA) was prepared.

  • MG:OVA caused the maturation of bone marrow derived dendritic cells (BMDC).

  • BMDC matured by MG:OVA activated naïve OVA-specific transgenic CD4 and CD8 T cells.

  • BMDC treated with OVA alone failed to activate naïve T cells.

  • Microparticulate β-glucan should be studied further as a potential vaccine adjuvant.

Abstract

Microparticulate β-glucan (MG) conjugated to vaccine antigen has been shown to serve as an effective adjuvant in vivo. To further study antigen presentation by MG:vaccine conjugates, bone marrow-derived dendritic cells (BMDC) were treated with MG conjugated to ovalbumin (OVA), then interacted with splenocytes from DO11.10 transgenic mice expressing an OVA peptide-specific T cell receptor. BMDC treated with MG:OVA induced significantly higher numbers of activated (CD25+CD69+) OVA-specific CD4+ T cells than BMDC treated with OVA alone. BMDC treated with MG:OVA upregulated CD86 and CD40 expression as well as MG alone, indicating that conjugation of OVA does not alter the immunostimulatory capacity of MG. Activation of CD8+ OVA-specific OT-1 cells showed that MG:OVA is also capable of enhancing cross-presentation by BMDC to CD8+ cytotoxic T cells. These results show that MG acts as an adjuvant to enhance antigen presentation by dendritic cells to naïve, antigen-specific CD4 and CD8 T cells.

Keywords

β-Glucan
Vaccine adjuvant
Dendritic cells
CD4 T cells
CD8 T cells

Cited by (0)