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Validation and Implementation of Marker-Assisted Selection (MAS) for PVY Resistance (Ry adg gene) in a Tetraploid Potato Breeding Program

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Abstract

The gene Ry adg from S. tuberosum ssp. andigena provides extreme resistance to PVY. This gene has been genetically mapped to chromosome XI and linked PCR-based DNA markers have been identified. Advanced tetraploid russeted potato clones developed by the U.S. Pacific Northwest Potato Breeding (‘Tri-State’) Program with Ry adg PVY resistance were used in this study. The objective of this work was to assess the usefulness of molecular markers linked to Ry adg as a tool for selecting PVY resistance in a tetraploid potato breeding program. To achieve this, a full-sib tetraploid population segregating for Ry adg was screened with molecular markers linked to Ry adg , artificially inoculated with PVYO and evaluated in the greenhouse. A large percentage (96.4%) of the segregating lines showed coincidence between molecular markers and ELISA results at 40 days after inoculation. This justifies the use of molecular markers as an alternative to artificial inoculation followed by ELISA. Segregation (resistant vs. susceptible) based on ELISA and molecular marker results in the full-sib population indicated the presence of Ry adg as a simplex in the PVY resistant parent OR00030-1. Additional full-sib populations segregating for the Ry adg gene coming from OR00030-1 and from a related clone, AOR00628-3, were evaluated under field conditions. MAS can be used as a fast and efficient tool to select for PVY resistance, reducing the number of PVY susceptible lines retained for succeeding field evaluations, and thereby increasing the odds of generating PVY resistant potato varieties.

Resumen

El gen Ry adg de S. tuberosum ssp. andígena ofrece resistencia extrema contra el virus Y de la papa (PVY). Este gen ha sido genéticamente mapeado en el cromosoma XI y se han identificado marcadores ligados de ADN basado en PCR (reacción en cadena de la polimerasa). Clones avanzados tetraploides de papa rugosas, desarrollados por el Programa de Mejoramiento del Pacífico Noroeste de los Estados Unidos (`Tri-State´) con resistencia Ry adg al PVY fueron utilizados en este estudio. El objetivo de este trabajo fue evaluar la utilidad de los marcadores moleculares ligados a Ry adg , como una herramienta para seleccionar resistencia al PVY en un programa de mejoramiento de papa tetraploide. Para lograr esto, una población tetraploide de hermanos completos, segregantes para Ry adg fue tamizada mediante marcadores moleculares ligados al Ry adg , inoculada artificialmente con PVYo y evaluada en invernadero. Un alto porcentaje (96.4%) de las líneas segregantes mostró coincidencia entre los marcadores moleculares y los resultados de ELISA 40 días después de la inoculación. Esto justifica el uso de marcadores moleculares como una alternativa a la inoculación artificial seguida por ELISA. La segregación (resistente vs. susceptible) basada en ELISA y los resultados de los marcadores moleculares en la población de hermanos completos, indicó la presencia de Ry adg como un simplex en el progenitor PVY resistente OR00030-1. Poblaciones adicionales de hermanos completos segregantes para el gen Ry adg y provenientes de OR00030-1 y de un clon relacionado, AOR00628-3, fueron evaluadas bajo condiciones de campo. MAS (selección asistida por marcadores) puede ser utilizada como una herramienta rápida y eficiente para seleccionar resistencia al PVY, reduciendo el número de líneas susceptibles al PVY retenidas para evaluaciones de campo futuras y así incrementar las probabilidades de generar variedades de papa resistentes al PVY.

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Acknowledgments

The Oregon Potato Commission and the USDA/CSREES Special Potato Research Grant program provided financial support for this research. The authors thank Dr. Oscar Gutbrod for providing advice to assess visual PVY symptoms in the greenhouse. Thanks are extended to Kandy Marling, Eda Karaağaç, and several undergrad students for technical assistance.

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Authors and Affiliations

  1. Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97331, USA

    Ryon J. Ottoman, Solomon Yilma & M. Isabel Vales

  2. Hermiston Agricultural Research and Extension Center, Oregon State University, Hermiston, OR, 97838, USA

    Dan C. Hane

  3. United States Department of Agriculture (USDA)-Agricultural Research Service (ARS), Prosser, WA, 99350, USA

    Charles R. Brown & James M. Crosslin

  4. Central Oregon Agricultural Research Center, Oregon State University, Madras, OR, 97741, USA

    Steven R. James

  5. Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97331, USA

    Alvin R. Mosley

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  1. Ryon J. Ottoman
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  2. Dan C. Hane
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  3. Charles R. Brown
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  4. Solomon Yilma
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  8. M. Isabel Vales
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Correspondence to M. Isabel Vales.

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Ottoman, R.J., Hane, D.C., Brown, C.R. et al. Validation and Implementation of Marker-Assisted Selection (MAS) for PVY Resistance (Ry adg gene) in a Tetraploid Potato Breeding Program. Am. J. Pot Res 86, 304–314 (2009). https://doi.org/10.1007/s12230-009-9084-0

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  • DOI: https://doi.org/10.1007/s12230-009-9084-0

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