Abstract
Chicken parvovirus (ChPV) has been associated with malabsorption syndrome (MAS) in broilers. However, the participation of this virus in such syndrome is unclear, since it may be detected in diseased and healthy chickens. In the course of these studies, it was argued whether ChPV genome loads might be correlated to the occurrence of MAS. To check such a hypothesis, a SYBR green-based quantitative polymerase chain reaction was developed to detect and quantify ChPV genomes. Cloacal swabs from 68 broilers with MAS and 59 from healthy animals were collected from different poultry farms. Genomes of ChPV were detected in all samples, regardless of their health status. However, viral genome loads in MAS-affected broilers were significantly higher (1 × 105 genome copies per 100 ng DNA) than in healthy animals (1.3 × 103 GC/100 ng DNA). These findings indicate that there is an association between high ChPV genome loads and the occurrence of MAS in broilers.
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Acknowledgments
This work was partially supported by the CNPq, FINEP (01.10.0783.04) and CAPES; F. Finkler has been supported by a CNPq Master Degree studentship. P.M. Roehe is a CNPq 1A research fellow; A.C. Franco is a CNPq 2 research fellow.
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This study was approved by the Ethics and Animal Experimentation Committee (CEUA) of FEPAGRO Saúde Animal – Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), project no. 04/14.
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Finkler, F., de Lima, D.A., Cerva, C. et al. Chicken parvovirus viral loads in cloacal swabs from malabsorption syndrome-affected and healthy broilers. Trop Anim Health Prod 48, 1685–1689 (2016). https://doi.org/10.1007/s11250-016-1144-0
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DOI: https://doi.org/10.1007/s11250-016-1144-0