Abstract
Distant hybridizations are important for developing common wheat germplasm. Thinopyrum ponticum (2n = 10x = 70), which is a wild relative of wheat, has numerous advantages for enhancing the tolerance of plants to biotic and abiotic stresses. ES-11 and ES-12 are two stable lines derived from a cross between the Triticum aestivum–Th. ponticum partial amphiploid line Xiaoyan784 (2n = 8x = 56) and the wheat Abbondanza nullisomic lines (2n = 40) involving consecutive self-crosses and cytological marker-assisted selection. Lines ES-11 and ES-12 were characterized by a cytogenetic analysis, a sequential fluorescence in situ hybridization (FISH)–genomic in situ hybridization (GISH), and a multicolor GISH (mc-GISH) combined with an analysis of functional molecular markers. Moreover, their agronomic traits and disease resistance were evaluated. The cytogenetic results suggested that ES-11 and ES-12 contained 42 chromosomes. In ES-12, wheat chromosome 3D was replaced by a pair of Th. ponticum 3St chromosomes for a genome composition of 14A + 14B + 12D + 2(3St). In ES-11, wheat chromosome 3B and 4D were replaced by chromosomes 3St and 4 J, respectively, for a genome composition of 14A + 12B + 12D + 2(3St) +2(4J). The detected recombination between chromosomes 3St and 4J and the structural variation of chromosome 2A were due to the introduction of two pairs of Th. ponticum chromosomes. Additionally, ES-11 and ES-12 were resistant to stripe rust at the seedling and adult stages. Thus, the highly disease-resistant wheat–Th. ponticum disomic substitution line (ES-12) and double substitution line (ES-11) are potentially useful germplasms for breeding disease-resistant wheat lines.
Similar content being viewed by others
References
Bariana HS, McIntosh RA (1993) Cytogenetic studies in wheat. XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome 36:476–482. https://doi.org/10.1139/g93-065
Chen GL, Zheng Q, Bao YG, Liu SB, Wang HG, Li XF (2012) Molecular cytogenetic identification of a novel dwarf wheat line with introgressed Thinopyrum ponticum chromatin. J Biosci 37:149–155. https://doi.org/10.1007/s12038-011-9175-1
Ciferri R (1955) The first interspecific wheat hybrids. J Heredity 46:81–83. https://doi.org/10.1093/oxfordjournals.jhered.a106529
Du P, Zhuang LF, Wang YZ, Yuan L, Wang Q, Wang DR, Dawadondup TLJ, Shen J, Xu HB, Zhao H, Chu CG, Qi ZJ (2017) Development of oligonucleotides and multiplex probes for quick and accurate identification of wheat and Thinopyrum bessarabicum chromosomes. Genome 60:93–103. https://doi.org/10.1139/gen-2016-0095
Fu SL, Lv ZL, Qi B, Guo X, Li J, Liu B, Han F (2012) Molecular cytogenetic characterization of wheat–Thinopyrum elongatum addition, substitution and translocation lines with a novel source of resistance to wheat Fusarium head blight. J Genet Genomics 39:103–110. https://doi.org/10.1016/j.jgg.2011.11.008
Grewal S, Yang C, Edwards SH, Scholefield D, Ashling S, Burridge AJ, King IP, King J (2018) Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat. Theor Appl Genet 131:389–406. https://doi.org/10.1007/s00122-017-3009-y
Guo J, Yu XC, Yin HY, Liu GJ, Li AF, Wang HW, Kong LR (2016) Phylogenetic relationships of Thinopyrum and Triticum species revealed by SCoT and CDDP markers. Plant Syst Evol 302:1301–1309. https://doi.org/10.1007/s00606-016-1332-4
Han FP, Liu B, Fedak G, Liu ZH (2004) Genomic constitution and variation in five partial amphiploids of wheat–Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. Theor Appl Genet 109:1070–1076. https://doi.org/10.1007/s00122-004-1720-y
He F, Xing PY, Bao YG, Ren MJ, Liu SB, Wang YH, Li XF, Wang HG (2017) Chromosome pairing in hybrid progeny between Triticum aestivum and Elytrigia elongata. Front Plant Sci 8:2161. https://doi.org/10.3389/fpls.2017.02161
Huang XY, Zhu MQ, Zhuang LF, Zhang SY, Wang JJ, Chen XJ, Wang DR, Chen JY, Bao YG, Guo J, Zhang JL, Feng YG, Chu CG, Du P, Qi ZJ, Wang HG, Chen PD (2018) Structural chromosome rearrangements and polymorphisms identified in Chinese wheat cultivars by high-resolution multiplex oligonucleotide FISH. Theor Appl Genet 131:1967–1986. https://doi.org/10.1007/s00122-018-3126-2
Kong LN, Song XY, Xiao J, Sun HJ, Dai KL, Lan CX, Singh P, Yuan CX, Zhang SZ, Singh R, Wang HY, Wang XE (2018) Development and characterization of a complete set of Triticum aestivum–Roegneria ciliaris disomic addition lines. Theor Appl Genet 131:1793–1806. https://doi.org/10.1007/s00122-018-3114-6
Kruppa K, Molnár-Láng M (2016) Simultaneous visualization of different genomes (J, JSt and St) in a Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid (Poaceae) and in its parental species by multicolour genomic in situ hybridization (mcGISH). Comp Cytogenet 10:283–293. https://doi.org/10.3897/CompCytogen.v10i2.7305
Kruppa K, Turkosi E, Mayer M, Toth V, Vida G, Szakacs E, Molnár-Láng M (2016) McGISH identification and phenotypic description of leaf rust and yellow rust resistant partial amphiploids originating from a wheat × Thinopyrum synthetic hybrid cross. J Appl Genet 57:427–437. https://doi.org/10.1007/s13353-016-0343-8
Li ZS, Li B, Tong YP (2008) The contribution of distant hybridization with decaploid Agropyron elongatum to wheat improvement in China. J Genet Genomics 35:451–456. https://doi.org/10.1016/S1673-8527(08)60062-4
Li AL, Geng SF, Zhang LQ, Liu DC, Mao L (2015a) Making the bread: insights from newly synthesized allohexaploid wheat. Mol Plant 8:847–859. https://doi.org/10.1016/j.molp.2015.02.016
Li H, Guo XX, Wang CY, Ji WQ (2015b) Spontaneous and divergent hexaploid triticales derived from common wheat x rye by complete elimination of D-genome chromosomes. PLoS One 10:e0120421. https://doi.org/10.1371/journal.pone.0120421
Linc G, Sepsi A, Molnár-Láng M (2012) A FISH karyotype to study chromosome polymorphisms for the Elytrigia elongata E genome. Cytogenet Genome Res 136:138–144. https://doi.org/10.1159/000334835
Liu B, Xu CM, Zhao N, Qi B, Kimatu JN, Pang JS, Han FP (2009) Rapid genomic changes in polyploid wheat and related species: implications for genome evolution and genetic improvement. J Genet Genomics 36:519–528. https://doi.org/10.1016/S1673-8527(08)60143-5
Liu G, Jia LJ, Lu LH, Qin DD, Zhang JP, Guan PF, Ni ZF, Yao YY, Sun QX, Peng HR (2014) Mapping QTLs of yield-related traits using RIL population derived from common wheat and Tibetan semi-wild wheat. Theor Appl Genet 127:2415–2432. https://doi.org/10.1007/s00122-014-2387-7
Liu SW, Li F, Kong LN, Sun Y, Qin LM, Chen SY, Cui HF, Huang YH, Xia GM (2015) Genetic and epigenetic changes in somatic hybrid introgression lines between wheat and tall wheatgrass. Genetics 199:1035–1045. https://doi.org/10.1534/genetics.114.174094
Niu Z, Klindworth DL, Yu G, Friesen TL, Chao S, Jin Y, Cai X, Ohm JB, Rasmussen JB, Xu SS (2014) Development and characterization of wheat lines carrying stem rust resistance gene Sr43 derived from Thinopyrum ponticum. Theor Appl Genet 127:969–980. https://doi.org/10.1007/s00122-014-2272-4
Pei YR, Cui Y, Zhang YP, Wang HG, Bao YG, Li XF (2018) Molecular cytogenetic identification of three rust-resistant wheat–Thinopyrum ponticum partial amphiploids. Mol Cytogenet 11:27–27. https://doi.org/10.1186/s13039-018-0378-0
Sepsi A, Molnar I, Szalay D, Molnár-Láng M (2008) Characterization of a leaf rust-resistant wheat–Thinopyrum ponticum partial amphiploid BE-1, using sequential multicolor GISH and FISH. Theor Appl Genet 116:825–834. https://doi.org/10.1007/s00122-008-0716-4
Tang ZX, Yang ZJ, Fu SL (2014) Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet 55:313–318. https://doi.org/10.1007/s13353-014-0215-z
Wang RRC, Lu BR (2014) Biosystematics and evolutionary relationships of perennial Triticeae species revealed by genomic analyses. J Syst Evol 52:697–705. https://doi.org/10.1111/jse.12084
Wang YH, Wang HW (2016) Characterization of three novel wheat–Thinopyrum intermedium addition lines with novel storage protein subunits and resistance to both powdery mildew and stripe rust. J Genet Genomics 43:45–48. https://doi.org/10.1016/j.jgg.2015.10.004
Wang YJ, Quan W, Peng NN, Wang CY, Yang XF, Liu XL, Zhang H, Chen CH, Ji WQ (2016) Molecular cytogenetic identification of a wheat–Aegilops geniculata Roth 7Mg disomic addition line with powdery mildew resistance. Mol Breed 36:1–10. https://doi.org/10.1007/s11032-016-0463-1
Yang ZJ, Li GR, Chang ZJ, Zhou JP, Ren ZL (2006) Characterization of a partial amphiploid between Triticum aestivum cv. Chinese Spring and Thinopyrum intermedium ssp trichophorum. Euphytica 149:11–17. https://doi.org/10.1007/s10681-005-9010-6
Yang XF, Wang CY, Chen CH, Zhang H, Tian ZR, Li X, Wang YJ, Ji WQ (2014) Chromosome constitution and origin analysis in three derivatives of Triticum aestivum–Leymus mollis by molecular cytogenetic identification. Genome 57:583–591. https://doi.org/10.1139/gen-2014-0161
Zhan HX, Li GR, Zhang XJ, Li X, Guo HJ, Gong WP, Jia JQ, Qiao LY, Ren YK, Yang ZJ, Chang ZJ (2014) Chromosomal location and comparative genomics analysis of powdery mildew resistance gene Pm51 in a putative wheat–Thinopyrum ponticum introgression line. PLoS One 9:e113455. https://doi.org/10.1371/journal.pone.0113455
Zhang XY, Dong YS, Wang RRC (1996) Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum x Thinopyrum ponticum by in situ hybridization, isozyme analysis, and RAPD. Genome 39:1062–1071. https://doi.org/10.1139/G96-133
Zhang P, Li WL, Fellers J, Friebe B, Gill BS (2004) BAC-FISH in wheat identifies chromosome landmarks consisting of different types of transposable elements. Chromosoma 112:288–299. https://doi.org/10.1007/s00412-004-0273-9
Zhang JP, Zhang P, Hewitt T, Li JB, Dundas I, Schnippenkoetter W, Hoxha S, Chen CH, Park R, Lagudah E (2019) A strategy for identifying markers linked with stem rust resistance in wheat harbouring an alien chromosome introgression from a non-sequenced genome. Theor Appl Genet 132:125–135. https://doi.org/10.1007/s00122-018-3201-8
Zheng Q, Lv ZL, Niu ZX, Li B, Li HW, Xu SS, Han FP, Li ZS (2014) Molecular cytogenetic characterization and stem rust resistance of five wheat–Thinopyrum ponticum partial amphiploids. J Genet Genomics 41:591–599. https://doi.org/10.1016/j.jgg.2014.06.003
Zheng Q, Luo QL, Niu ZX, Li HW, Li B, Xu SS, Li ZS (2015) Variation in chromosome constitution of the Xiaoyan series partial Amphiploids and its relationship to stripe rust and stem rust resistance. J Genet Genomics 42:657–660. https://doi.org/10.1016/j.jgg.2015.08.004
Zhu C, Wang YZ, Chen CH, Wang CY, Zhang AC, Peng NN, Wang YJ, Zhang H, Liu XL, Ji WQ (2017) Molecular cytogenetic identification of a wheat–Thinopyrum ponticum substitution line with stripe rust resistance. Genome 60:860–867. https://doi.org/10.1139/gen-2017-0099
Acknowledgments
This work was supported by the National Key Research and Development Program of China (grant no. 2016YFD0102000), and the Zhongying Tang Breeding Foundation of Northwest A&F University. We are grateful for their financial support.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
ESM 1
(DOCX 23 kb)
Rights and permissions
About this article
Cite this article
Wang, S., Wang, C., Wang, Y. et al. Molecular cytogenetic identification of two wheat–Thinopyrum ponticum substitution lines conferring stripe rust resistance. Mol Breeding 39, 143 (2019). https://doi.org/10.1007/s11032-019-1053-9
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11032-019-1053-9