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Cloning of TaTPP-6AL1 associated with grain weight in bread wheat and development of functional marker

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Abstract

Trehalose 6-phosphate phosphatase (TPP) dephosphorylates trehalose 6-phosphate to trehalose, an important growth regulator, and is involved in starch accumulation and grain yield. In this study, wheat TPP homologs were isolated from chromosomes 6AL, 6BL, and 6DL, designated as TaTPP-6AL1, TaTPP-6BL1, and TaTPP-6DL1, respectively. Sequence alignment showed a single-nucleotide polymorphism (SNP) at TaTPP-6AL1 locus between cultivars with contrasting thousand grain weight (TGW), forming alleles TaTPP-6AL1a and TaTPP-6AL1b, respectively. A cleaved amplified polymorphic sequence (CAPS) marker, TaTPP-6AL1-CAPS, was developed to differentiate the two alleles. TaTPP-6AL1 was mapped within the interval of IWB65749 and IWB60449 in a recombinant inbred line (RIL) population derived from Zhou8425B/Chinese Spring using the wheat 90K SNP assay. A QTL for TGW identified in the interval explained 12.1–19.1% of the phenotypic variance across five environments. Association analysis on 141 Chinese wheat cultivars also indicated a significant correlation of TaTPP-6AL1 with TGW. In conclusion, TaTPP-6AL1 and its functional marker are valuable to improve grain yield in wheat breeding.

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Acknowledgement

This study was supported by the National Key Research and Development Program of China (2016YFD0101802, 2016YFE0108600) and the National Natural Science Foundation of China (31461143021, 31571663).

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Authors and Affiliations

  1. College of Agronomy, Henan University of Science and Technology, Luoyang, Henan, 471023, China

    Pengfei Zhang & Chunping Wang

  2. Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, China

    Pengfei Zhang, Zhonghu He, Xiuling Tian, Fengmei Gao, Dengan Xu, Jindong Liu, Weie Wen, Luping Fu, Xianchun Xia & Shuanghe Cao

  3. International Maize and Wheat Improvement Center (CIMMYT) China Office, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Zhonghu He

  4. Keshan Sub-Academy, Heilongjiang Academy of Agricultural Sciences, Keshan, Heilongjiang, 161606, China

    Fengmei Gao

  5. Crop Research Institute, Shandong Academy of Agricultural Sciences, 202 Gongye Bei Road, Jinan, Shandong, 250100, China

    Genying Li & Xinxia Sui

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  1. Pengfei Zhang
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  2. Zhonghu He
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  9. Genying Li
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  13. Shuanghe Cao
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Correspondence to Chunping Wang or Shuanghe Cao.

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Zhang, P., He, Z., Tian, X. et al. Cloning of TaTPP-6AL1 associated with grain weight in bread wheat and development of functional marker. Mol Breeding 37, 78 (2017). https://doi.org/10.1007/s11032-017-0676-y

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