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Effect of insulin-like growth factor-1 (IGF-1) on the gluconeogenesis in calf hepatocytes cultured in vitro

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Abstract

The major role of insulin-like growth factor-1 (IGF-1) in the liver is to mediate glucose uptake in hepatocytes to synthesize glycogen and maintain blood glucose homeostasis. In this study, to evaluate the role of IGF-1 on gluconeogenesis and nutrient metabolism in dairy cattle, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) expression and enzyme activity were evaluated in primary cultures of bovine hepatocytes treated with different concentrations of IGF-1 by quantitative polymerase chain reaction and spectrophotometry, respectively. The results showed that expression of PC and PEPCK were significantly lower in bovine hepatocytes by IGF-1 treatment in test group compare to the control group (P < 0.01). As IGF-1 concentration increased, PC and PEPCK enzyme activity in bovine hepatocytes decreased. Evaluating PC and PEPCK mRNA levels and enzyme activity may thus be useful to monitor subclinical ketosis in dairy cows.

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References

  1. Castaeda-Gutierrez E, Pelton S, Gilbert R, Butler W (2009) Effect of peripartum dietary energy supplementation of dairy cows on metabolites, liver function and reproductive variables. Anim Reprod Sci 112(3–4):301–315

    Article  Google Scholar 

  2. Drackley JK (1999) Biology of dairy cows during the transition period: the final frontier? J Dairy Sci 82:2259–2273

    Article  PubMed  CAS  Google Scholar 

  3. Pedernera M, Celi P, Garcia SC, Salvin HE, Barchia I, Fulkerson WJ (2010) Effect of diet, energy balance and milk production on oxidative stress in early-lactating dairy cows grazing pasture. Vet J 186(3):352–357

    Article  PubMed  CAS  Google Scholar 

  4. Ingvartsen KL (2006) Feeding-and management-related diseases in the transition cow: physiological adaptations around calving and strategies to reduce feeding-related diseases. Anim Feed Sci Tech 126(3–4):175–213

    Article  Google Scholar 

  5. Liu GW, Zhang ZG, Wang JG, Wang Z, Xu C, Zhu XL (2010) Insulin receptor gene expression in normal and diseased bovine liver. J Comp Pathol 143(4):258–261

    Article  PubMed  CAS  Google Scholar 

  6. Croniger CM, Olswang Y, Reshef L, Kalhan SC, Tilghman SM, Hanson RW (2002) Phosphoenolpyruvate carboxykinase revisited: insights into its metabolic role. Biochem Mol Biol Edu 30(1):14–20

    Article  CAS  Google Scholar 

  7. Greenfield RB, Cecava MJ, Donkin SS (2000) Changes in mRNA expression for gluconeogenic enzymes in liver of dairy cattle during the transition to lactation. J Dairy Sci 83(6):1228–1236

    Article  PubMed  CAS  Google Scholar 

  8. Andersson L (1988) Subclinical ketosis in dairy cows. Vet Clin North Am Food Anim Pract 4(2):233–251

    PubMed  CAS  Google Scholar 

  9. Velez J, Donkin S (2005) Feed restriction induces pyruvate carboxylase but not phosphoenolpyruvate carboxykinase in dairy cows. J Dairy Sci 88(8):2938–2948

    Article  PubMed  CAS  Google Scholar 

  10. Rhoads M, Meyer J, Kolath S, Lamberson W, Lucy M (2008) Growth hormone receptor, insulin-like growth factor (IGF)-1, and IGF-binding protein-2 expression in the reproductive tissues of early postpartum dairy cows. J Dairy Sci 91(5):1802–1813

    Article  PubMed  CAS  Google Scholar 

  11. McCarthy S, Butler S, Patton J, Daly M, Morris D, Kenny D, Waters S (2009) Differences in the expression of genes involved in the somatotropic axis in divergent strains of Holstein-Friesian dairy cows during early and mid lactation. J Dairy Sci 92(10):5229–5238

    Article  PubMed  CAS  Google Scholar 

  12. Feng B, Li J, Kliegman RM (1996) Differential effects of insulin-like growth factor-1 on neonatal canine gene expression. Biochem Mol Med 59(2):154–160

    Article  PubMed  CAS  Google Scholar 

  13. Zhang ZG, Li XB, Gao L, Liu GW, Kong T, Li YF, Wang HB, Zhang C, Wang Z, Zhang RH (2011) An updated method for the isolation and culture of primary calf hepatocytes. Vet J doi:10.1016/j.tvjl.2011.01.008

  14. Hirai C, Ichiba H, Saito M, Shintaku H, Yamano T, Kusuda S (2002) Trophic effect of multiple growth factors in amniotic fluid or human milk on cultured human fetal small intestinal cells. J Pediatr Gastr Nutr 34(5):524

    Article  Google Scholar 

  15. Andree AD, Marc P (2006) Rapid and simple comparison of messenger RNA levels using real-time PCR. Biol Proced 8(1):1–10

    Google Scholar 

  16. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45

    Article  PubMed  CAS  Google Scholar 

  17. Li XW, Li XB, Bai G, Chen H, Deng QH, Liu ZX, Zhang L, Liu GW, Wang Z (2011) Effect of non-esterified fatty acids on the gluconeogenesis in bovine hepatocytes. Mol Cell Biochem DOI 10.1007/s110100111032x

  18. Hammon HM, Sauter SN, Reist M, Zbinden Y, Philipona C, Morel C, Blum JM (2003) Dexamethasone and colostrum feeding affect hepatic gluconeogenic enzymes differently in neonatal calves. J Anim Sci 81(12):3095–3106

    PubMed  CAS  Google Scholar 

  19. Agca C, Greenfield RB, Hartwell JR, Donkin SS (2002) Cloning and characterization of bovine cytosolic and mitochondrial PEPCK during transition to lactation. Physiol Genomics 11(2):53–63

    PubMed  CAS  Google Scholar 

  20. Kabir G, Hossain M, Faruque MO, Hassan N, Hassan Z, Nahar Q, Shefin SM, Alauddin M, Ali L (2010) Association of serum free IGF-1 and IGFBP-1 with insulin sensitivity in impaired glucose tolerance (IGT). Int J Diabetes Mellitus 2(3):144–147

    Article  Google Scholar 

  21. Sirotkin A (2005) Control of reproductive processes by growth hormone: extra-and intracellular mechanisms. Vet J 170(3):307–317

    Article  PubMed  CAS  Google Scholar 

  22. Di Cola G, Cool MH, Accili D (1997) Hypoglycemic effect of insulin-like growth factor-1 in mice lacking insulin receptors. J Clin Invest 99(10):2538

    Article  PubMed  CAS  Google Scholar 

  23. Giovanni DC, Martha HC (1997) Hypoglycemic effect of insulin-like growth factor-1 in mice lacking insulin receptors. J Clin Invest 99:2538–2544

    Article  Google Scholar 

  24. Velez J, Donkin S (2004) Bovine somatotropin increases hepatic phosphoenolpyruvate carboxykinase mRNA in lactating dairy cows. J Dairy Sci 87(5):1325–1335

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by the National Key Basic Research Program of China (Grant No. 2011CB100800), by the National Natural Science Foundation of China (Grant Nos. 31072178, 30972212, and 30871897), by the Science Fund for Distinguished Young Scholars of Jilin University (Grant No. 201100009).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, 130062, China

    Jianguo Wang, Xiaoyan Zhu, Xiaobing Li, Peng Li, Zhe Wang & Guowen Liu

  2. Laboratory Animal Center, Jilin University, Changchun, 130062, China

    Chengzhen Chen & Yan Gao

  3. College of Animal Science and Veterinary Medicine, Shenyang Agriculture University, Shenyang, 110161, China

    Peng Li, Yi Zhang & Miao Long

Authors
  1. Jianguo Wang
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  2. Xiaoyan Zhu
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  3. Chengzhen Chen
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  4. Xiaobing Li
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  5. Yan Gao
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  6. Peng Li
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  7. Yi Zhang
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  8. Miao Long
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  9. Zhe Wang
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  10. Guowen Liu
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Corresponding authors

Correspondence to Zhe Wang or Guowen Liu.

Additional information

Jianguo Wang, Xiaoyan Zhu and Chengzhen Chen contributed equally to this study.

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Wang, J., Zhu, X., Chen, C. et al. Effect of insulin-like growth factor-1 (IGF-1) on the gluconeogenesis in calf hepatocytes cultured in vitro. Mol Cell Biochem 362, 87–91 (2012). https://doi.org/10.1007/s11010-011-1130-9

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  • DOI: https://doi.org/10.1007/s11010-011-1130-9

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