Abstract
Objective
RNAe is a new method that enhances protein expression at the post-transcriptional level. RNAe utility was further explored to improve endogenous protein expression.
Results
Transgenic mice were created by targeting RNAe to growth hormone gene into the C57/BL mouse genome by transposon mediated integration; the mice showed a heavier body weight and longer body length compared with normal mice. RNAe can also be used for gene therapy through the delivery of in vitro transcribed RNA.
Conclusion
This study takes a further step towards applying RNAe in pharmaceutical approaches by transposon-based transgenic mice model construction and the use of in vitro transcribed RNA transfection assay.
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Acknowledgments
This research was supported by Natural Sciences Foundation of China (Grant Nos. 31170940, 31470933), 973 Basic Research Fund (Grant No. 2012CB725204), National High Technology Research and Development (863 Program) (Grant Nos. 2012AA020503, 2013AA020301 and 2012AA02A702), Tsinghua University Initiative Scientific Research Program (No. 20131089199).
Supporting information
Supplementary Fig. 1—Map of the pPiggy-RNAe-mGH-polyA plasmid.
Supplementary Fig. 2—Growth curve for the RNAe-mGH transgenic mice over 30 weeks (the deceased mice were included).
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Haizhou Long and Yi Yao are contributed equally to this work.
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Long, H., Yao, Y., Jin, S. et al. RNAe in a transgenic growth hormone mouse model shows potential for use in gene therapy. Biotechnol Lett 39, 179–188 (2017). https://doi.org/10.1007/s10529-016-2236-7
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DOI: https://doi.org/10.1007/s10529-016-2236-7