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Nocardioides acrostichi sp. nov., a novel endophytic actinobacterium isolated from leaf of Acrostichum aureum

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Abstract

A short-rod-shaped, non-spore-forming endophytic actinobacterium, was isolated from a surface-sterilized leaf of Acrostichum aureum in Fangchenggang, Guangxi Zhuang Autonomous Region, China, designated strain CBS4Y-1T and examined by a polyphasic approach to determine its taxonomic position. This actinobacterium was Gram-staining-positive and aerobic. Substrate mycelia and aerial mycelia were not observed, and no diffusible pigments were observed on the media tested. Strain CBS4Y-1T grew optimally with 0–1.0% (w/v) NaCl at 30 °C, pH 7.0–8.0. Comparative analysis of 16S rRNA genes showed that strain CBS4Y-1T shared the highest 16S rRNA gene sequence similarities with Nocardioides marinus CL-DD14T (96.7%) and Nocardioides terrae BX5-10T (96.7%). Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CBS4Y-1T belonged to the genus Nocardioides and formed a distinct cluster within the genus Nocardioides. The DNA G + C content of strain CBS4Y-1T was 71.1 mol%. The cell-wall peptidoglycan contained LL-diaminopimelic acid and MK-8(H4) was the predominant menaquinone. Phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI) were detected in the polar lipid extracts. The major fatty acids were iso-C16:0, C18:1ω9c and iso-C17:0. On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics, strain CBS4Y-1T represents a novel species of the genus Nocardioides, for which the name Nocardioides acrostichi sp. nov. is proposed. The type strain is CBS4Y-1T (= KCTC 49238T = CGMCC 4.7548T).

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Data availability

All data generated or analysed during this study are included in this published article, its supplementary information files and GenBank/EMBL/DDBJ.

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Funding

This research was supported by the National Natural Sciences Foundation of China (NSFC, Grant No. 81960642), the Opening Project of Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry (No. GXMNPC2020002), Science and Technology Foundation of Guizhou Province (No. Qian Ke He Jichu [2019]1347) and Undergraduate Training Program for Innovation and Entrepreneurship of Guizhou Province (S202010661010).

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Authors and Affiliations

  1. Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, People’s Republic of China

    Xiao-Hui Chen, Ming-Sheng Chen, Xiao-Rui Yan, Zi-Bang He, Shun-Lian Li, You-Lang Wu & Li Tuo

  2. Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, 530007, People’s Republic of China

    Fei Li

  3. Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Disease, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, 100045, People’s Republic of China

    Fei-Na Li

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  1. Xiao-Hui Chen
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Contributions

X-HC performed genomic and phylogenetic analysis, wrote the manuscript. FL, F-NL and LT collected plant samples, performed chemotaxonomic analysis. M-SC and X-RY performed physiological and morphological analysis of the strain. Z-BH, S-LL and Y-LW isolated the strain and performed preliminary identification. LT conceived and designed the experiments and approved the final manuscript.

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Correspondence to Li Tuo.

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Chen, XH., Li, F., Li, FN. et al. Nocardioides acrostichi sp. nov., a novel endophytic actinobacterium isolated from leaf of Acrostichum aureum. Antonie van Leeuwenhoek 114, 479–486 (2021). https://doi.org/10.1007/s10482-021-01535-5

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