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Marinobacter profundi sp. nov., a slightly halophilic bacterium isolated from a deep-sea sediment sample of the New Britain Trench

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Abstract

A piezotolerant, cold-adapted, slightly halophilic bacterium, designated strain PWS21T, was isolated from a deep-sea sediment sample collected from the New Britain Trench. Cells were observed to be Gram-stain negative, rod-shaped, oxidase- and catalase-positive. Growth of the strain was observed at 4–45 °C (optimum 37 °C), at pH 5.0–9.0 (optimum 7.0) and in 0.5–20% (w/v) NaCl (optimum 3–4%). The optimum pressure for growth was 0.1 MPa (megapascal) with tolerance up to 70 MPa. 16S rRNA gene sequence analysis showed that strain PWS21T is closely related to Marinobacter guineae M3BT (98.4%) and Marinobacter lipolyticus SM19T (98.2%). Multilocus sequence analysis (MLSA) based on sequences of housekeeping genes gyrB, recA, atpD, rpoB and rpoD indicates that strain PWS21T represents a distinct evolutionary lineage within the genus Marinobacter. Furthermore, strain PWS21T showed low ANI and diDDH values to the closely related species. The principal fatty acids were identified as C12:0, C12:0 3-OH, C16:1ω9c, C16:0 and C18:1ω9c. Ubiquinone-9 was identified as the major respiratory quinone. The polar lipids were identified as phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), aminophospholipid (APL), two unidentified lipids and an unidentified phospholipid (PL). The G + C content of the genomic DNA was determined to be 60.3 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, we conclude that strain PWS21T represents a novel species of the genus Marinobacter, for which the name Marinobacter profundi sp. nov. is proposed (type strain PWS21T = KCTC 52990T = MCCC 1K03345T).

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Abbreviations

MCCC:

Marine Culture Collection of China

KCTC:

Korean Collection for Type Cultures

NCBI:

National Center for Biotechnology Information

diDDH:

The digital DNA–DNA hybridization

ANI:

The average nucleotide identity

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Acknowledgements

This work was supported by National Key R&D Program of China (Grant No. 2018YFC0310600) and by the National Natural Science Foundation of China (41773069, 41706146, 91328208, 41373071, and 41673085). The authors are grateful to all crews and on-board scientists of the M/V Zhangjian for taking the sediment samples in the New Britain Trench in September 2016.

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Authors and Affiliations

  1. Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Junwei Cao, Ping Liu, Yuli Wei, Rulong Liu & Jiasong Fang

  2. National Engineering Research Center for Oceanic Fisheries, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Junwei Cao, Ping Liu & Yuli Wei

  3. Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, People’s Republic of China

    Renju Liu

  4. State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan, 250100, People’s Republic of China

    Hainan Su

  5. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, People’s Republic of China

    Jiasong Fang

  6. Department of Natural Sciences, Hawaii Pacific University, Honolulu, HI, 96813, USA

    Jiasong Fang

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  1. Junwei Cao
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Cao, J., Liu, P., Liu, R. et al. Marinobacter profundi sp. nov., a slightly halophilic bacterium isolated from a deep-sea sediment sample of the New Britain Trench. Antonie van Leeuwenhoek 112, 425–434 (2019). https://doi.org/10.1007/s10482-018-1176-8

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