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Terrimonas suqianensis sp. nov., isolated from a tetrabromobisphenol A-contaminated soil

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Abstract

Strain C3-5T, a Gram-negative, asporogenous, rod-shaped bacterium, was isolated from a tetrabromobisphenol A contaminated soil. Growth was observed at 10–37 °C (optimum 30 °C) and at pH 5.5–9.5 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain C3-5T is a member of the genus Terrimonas and exhibits high sequence similarities with Terrimonas pekingensis QHT (99.0%) and Terrimonas rhizosphaerae CR94T (97.3%), and exhibits low (<97%) sequence similarities with other known Terrimonas species. Chemotaxonomic analysis revealed that strain C3-5T possesses menaquinone-7 (MK-7) as the major isoprenoid quinone and iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c) as the major (>5% of total) fatty acids. The polar lipids were determined to be a lipid, glycolipid, phospholipid, phosphoaminolipid and phosphatidylethanolamine. The DNA G+C content was found to be 42.6 mol%. The DNA–DNA relatedness values with the closely related strains T. pekingensis QHT and T. rhizosphaerae CR94T were 25.2 and 48.5%, respectively. Based on phylogenetic analysis, phenotypic characteristics and chemotaxonomic data, strain C3-5T is considered to represent a novel species of the genus Terrimonas, for which the name Terrimonas suqianensis sp. nov. is proposed. The type strain is C3-5T (= CCTCC AB 2017042T = KCTC 52676T).

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Abbreviations

CCTCC:

China Center for Type Culture Collection

CICC:

China Center of Industrial Culture Collection

KACC:

Kankakee Area Career Center

KCTC:

Korean Collection for Type Cultures

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Acknowledgements

This work was financially supported through grants from the National Natural Science Foundation of China (31400105; 31600080), the China Postdoctoral Science Foundation (2015T80562; 2014M561666) and the Natural Science Foundation of Shandong Province, China (ZR2016CB29).

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The authors declare that there are no conflicts of interest related to this paper.

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Authors and Affiliations

  1. Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Qing Chen, Xiao-Xia Zang, Xing Hang, Shun-Peng Li, Jian-Dong Jiang & Kai Chen

  2. College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, People’s Republic of China

    Qing Chen, Jing Chen & Hong-Mei Wang

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  1. Qing Chen
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  2. Xiao-Xia Zang
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Correspondence to Kai Chen.

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Chen, Q., Zang, XX., Hang, X. et al. Terrimonas suqianensis sp. nov., isolated from a tetrabromobisphenol A-contaminated soil. Antonie van Leeuwenhoek 110, 1061–1068 (2017). https://doi.org/10.1007/s10482-017-0879-6

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