Abstract
An alkalitolerant, thermotolerant, strictly aerobic and Gram-staining negative bacterial strain, designated YIM 78140T, was isolated from a water sample in Hehua hot spring, Tengchong, Yunnan province, south-west China. The colonies were light brown, convex and circular. Phylogenetic analysis of the 16S rRNA gene sequence of strain YIM 78140T indicated that it was clustered with members of β-Proteobacteria (with the similarity from 96.9 to 93.6 %). Good growth occurred at 40–50 °C, pH 8.0–9.0 and in the presence of 0–3 % (w/v) NaCl. The predominant ubiquinones were Q-8 and Q-9. The major fatty acids were C16:0, C17:0 cyclo, C18:1 ω7c and summed feature 3. The G+C content of genomic DNA was 70.8 mol%. The results of physiological and biochemical characteristics, phylogenetic analysis allowed the phenotypic and genotypic differentiation of strain YIM 78140T from its closest phylogenetic neighbours. Therefore, the strain YIM 78140T represents a novel genus of the family Comamonadaceae, for which the name Zhizhongheella caldifontis gen. nov., sp. nov. is proposed. The type strain is YIM 78140T (= BCRC 80649T = KCTC 32557T).
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Acknowledgments
The authors are grateful to Prof. Dr. Takuji Kudo (JCM) and Mrs. Min Tseng (BCRC) for their kind providing reference type strains. This research was supported by the Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. 2013DFA31980), National Science Foundation grant (OISE-0968421) and Key Project of Yunnan Provincial Natural Science Foundation (2013FA004).
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Lei Dong and Hong Ming contributed equally to this work.
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Dong, L., Ming, H., Liu, L. et al. Zhizhongheella caldifontis gen. nov., sp. nov., a novel member of the family Comamonadaceae . Antonie van Leeuwenhoek 105, 755–761 (2014). https://doi.org/10.1007/s10482-014-0131-6
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DOI: https://doi.org/10.1007/s10482-014-0131-6