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Marinobacter albus sp. nov., Isolated from Sand Sediment in a Coastal Intertidal Zone

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Abstract

A Gram-stain-negative bacterium with a rod-to-ovoid shape, named strain M216T, was isolated from sand sediment from the coastal intertidal zone of Huludao, Liaoning Province, China. Growth was observed at 8–40 °C (optimal, 30 °C), pH 5.5–9.5 (optimal, pH 6.5) and 0.5–14.0% (w/v) NaCl (optimal, 6%). Strain M216T possessed ubiquinone-9 as its sole respiratory quinone and phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophosphoglycolipid, one unidentified aminophospholipid, two unidentified phosphoglycolipids, three unidentified phospholipids and three unidentified glycolipids as the main polar lipids. C12:0, C16:0, C12:0 3-OH, C16:1 ω9c, C18:1 ω9c and summed features 3 (C16:1 ω7c and/or C16:1 ω6c) were the major fatty acids (> 5%). The 16S rRNA gene sequence of strain M216T exhibited high similarity to those of ‘Marinobacter arenosus’ CAU 1620T and Marinobacter adhaerens HP15T (99.3% and 98.5%, respectively) and less than 98.5% similarity to those of the other type strains. The ANI and dDDH values between the strain M216T and ‘Marinobacter arenosus’ CAU 1620T were 87.4% and 33.3%, respectively; these values were the highest among the other type strains but lower than the species threshold. The G+C content of strain M216T was 58.3%. Genomic analysis revealed that strain M216T harbors the major CAZymes of GH13, GH23, GH73, and PL5, which are responsible for polysaccharide degradation and the potential ability to reduce nitrate to ammonia. Through phenotypic, genotypic, and chemotaxonomic analyses, we proposed the name Marinobacter albus sp. nov., a novel species in the genus Marinobacter, with its type strain M216T (= MCCC 1K08600T = KCTC 82894T).

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Abbreviations

MA:

Marine agar

ANI:

Average nucleotide identity

DDH:

DNA‒DNA hybridization

MB:

Marine broth

ML:

Maximum likelihood

NJ:

Neighbour joining

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Funding

This study was supported by Zhejiang Provincial Natural Science Foundation of China (Grant No. LDT23D06025D06), the Key R&D Program of Zhejiang (Grant No. #2023C03011), National Science and Technology Fundamental Resources Investigation Program of China (Grant No. 2019FY100700) and National Natural Science Foundation of China (Grant No. 31900003).

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Authors and Affiliations

  1. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, People’s Republic of China

    Tai-Hang Deng, Yu Zhang, Jia-Wei Gao, Jun-Jie Ying, Lin Xu & Cong Sun

  2. Zhejiang Sci-Tech University Shaoxing Academy of Biomedicine Co., Ltd., Shaoxing, 312369, People’s Republic of China

    Tai-Hang Deng, Yu Zhang, Jia-Wei Gao, Jun-Jie Ying, Lin Xu & Cong Sun

  3. Trend Biotech Co., Ltd., Hangzhou, 311121, People’s Republic of China

    Wen-Wu Zhang & Yue Su

  4. Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, 310012, People’s Republic of China

    Xiao-Ming Xia

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  1. Tai-Hang Deng
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Contributions

Tai-Hang Deng: Conceptualization, Methodology, Writing–original draft, Formal analysis, Data curation, Visualization. Yu Zhang: Data curation, Visualization. Wen-Wu Zhang and Yue Su: Methodology, Formal analysis. Jia-Wei Gao and Jun-Jie Ying: Methodology, Data curation. Lin Xu and Xiao-Ming Xia: Conceptualization, Funding acquisition. Cong Sun: Conceptualization, Methodology, Writing–original draft, Supervision, Project administration, Funding acquisition.

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Correspondence to Cong Sun.

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Deng, TH., Zhang, Y., Zhang, WW. et al. Marinobacter albus sp. nov., Isolated from Sand Sediment in a Coastal Intertidal Zone. Curr Microbiol 81, 138 (2024). https://doi.org/10.1007/s00284-024-03676-y

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