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Bacillus arachidis sp. nov., Isolated from Peanut Rhizosphere Soil

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Abstract

A novel facultatively aerobic bacterium designated SY8 was isolated from a peanut rhizosphere soil sample collected in Jiangsu Province, China. Cells are Gram-stain-positive, rod-shaped, and agar colonies are creamy, opaque, and usually rhizoidal. Strain growth occurs at 30 – 45 °C (optimum 30 °C), pH 4.0 – 10.0 (optimum pH 6.0) and 0 – 4% (w/v) NaCl (optimum 2%) in Luria–Bertani medium. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain SY8 forms a distinct lineage in the clade of genus Bacillus and is related to Bacillus pseudomycoides DSM 12442 T (99.9%). Phylogenetic analysis of the concatenated gene sequences of 16S rRNA, gryB and rpoD also indicated that strain SY8 forms a distinct lineage in Bacillus. Calculation of the average nucleotide identities and the digital DNA–DNA hybridization values between strain SY8 and the related type Bacillus strains further revealed that strain SY8 represents a distinct species. The predominant cellular fatty acids are iso C15:0 (28.7%) and summed feature 3 (C16:1ω7c and/or C16:1ω6c) (10.3%). The major polar lipids consisted of diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidylinositol, and three unidentified phospholipids. The major menaquinone of SY8 was MK-7. Based on phenotypic, phylogenetic, chemotaxonomic, and genomic features, strain SY8 represents a novel species of the genus Bacillus. The name Bacillus arachidis sp. nov. is proposed with strain SY8T (= CCTCC AB 2021100 T=LMG 32409 T) designated as the type strain.

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Data Availability

Strain SY8T = CCTCC AB 2021100 T = LMG 32409 T. The GenBank accession number for the 16S rRNA gene and the whole-genome sequences of strain SY8T are OM062591 and JAGDQJ000000000, respectively.

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Acknowledgements

This research was funded by the National Natural Science Foundation of China (Grant No. 31771946).

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Authors and Affiliations

  1. Center for Bioengineering and Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China

    Yong Chen, Yang Li, Jie Shen, Qingxue Liu, Yuhang Liu, Yaqi Chu & Zijun Xiao

  2. School of Basic Medicine, Weifang Medical University, Weifang, 261042, China

    Yong Chen

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  1. Yong Chen
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  2. Yang Li
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  3. Jie Shen
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  4. Qingxue Liu
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  7. Zijun Xiao
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Contributions

Conceived and designed the experiments: Z. Xiao. Performed the experiments: Y. Li, J. Shen, Q. Liu, Y. Liu, Y. Chu. Analyzed the data: Y. Chen, Z. Xiao. Contributed reagents/materials/analysis tools: Z. Xiao. Wrote the paper: Y. Chen, Z. Xiao.

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Correspondence to Zijun Xiao.

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Chen, Y., Li, Y., Shen, J. et al. Bacillus arachidis sp. nov., Isolated from Peanut Rhizosphere Soil. Curr Microbiol 79, 231 (2022). https://doi.org/10.1007/s00284-022-02925-2

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