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Paenibacillus glycinis sp. nov., an Endophytic Bacterium Isolated from the Nodules of Soybean (Glycine max (L.) Merr)

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Abstract

A Gram-negative-staining, endospore-forming, rod-shaped bacterium, designated T1T, was isolated from root nodules of soybean (Glycine max (L.) Merr) in Heilongjiang Province of China. The isolate was identified as a member of the genus Paenibacillus based on phenotypic and phylogenetic characteristics. The 16S rRNA sequence was closely related to that of Paenibacillus sacheonensis SY01T with a similarity of 98.4%. Average nucleotide identity and in silico DNA–DNA hybridization values between strain T1T and P. sacheonensis DSM 23054 T were 81.4% and 25.4%, respectively. The DNA G + C content of strain T1T was 58.2 mol%. meso-Diaminopimelic acid was detected in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and iso-C15:0. The predominant respiratory quinone was MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified phospholipids, four unidentified aminophospholipids, an unidentified glycolipid and an unidentified lipid. Based on these results, T1T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus glycinis sp. nov. is proposed. The type strain is T1T (= CGMCC 1.18563 = KCTC43227).

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Abbreviations

ANI:

Average nucleotide identity

isDDH:

In silico DNA-DNA hybridization

FAME:

Fatty acid methyl esters

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31700003), “Academic Backbone” Project of Northeast Agricultural University (Grant No. 19XG15), Innovation Project of Hebei Academy of Agriculture and Forestry Sciences (2019-2-5 and 2019-1-4) and the Heilongjiang Postdoctoral Financial Assistance (Grant No. LBH-Z14042).

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  1. Hao Wang and Dong Hu contribute equally to this work.

Authors and Affiliations

  1. College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, People’s Republic of China

    Hao Wang, Ziqi Wang, Chunwei Yang, Siyuan Zhu & Chuntao Gu

  2. Key Laboratory of Plants Genetic Engineering Center, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, Hebei, People’s Republic of China

    Dong Hu

  3. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, Cd. México, México

    En Tao Wang

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The GenBank/ENA/DDBJ accession number for the 16S rRNA gene sequence of strain T1T is MT318143. The GenBank/ENA/DDBJ accession numbers for whole genome sequences of strain T1T and Paenibacillus sacheonensis DSM 23054T are JAAAMV000000000 and JAAAMU000000000, respectively.

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Wang, H., Hu, D., Wang, Z. et al. Paenibacillus glycinis sp. nov., an Endophytic Bacterium Isolated from the Nodules of Soybean (Glycine max (L.) Merr). Curr Microbiol 78, 1678–1685 (2021). https://doi.org/10.1007/s00284-021-02403-1

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