Abstract
A urea-utilizing bacterium, designated Q2-2 T, was isolated from landfill. Cells of strain Q2-2 T were Gram stain-negative, aerobic, short-rod bacteria. Strain Q2-2 T was observed to grow at a temperature range of 15–37℃ (optimum 30 ℃), a pH range of 5.5–9.5 (optimum pH 8.0) and 0–4% (w/v) NaCl (optimum 1%). The major respiratory quinone was Q-8, and the major polar lipids were diphosphatidyl glycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, and phosphatidyl glycerol. Based on the 16S rRNA gene sequence, strain Q2-2 T had the highest similarity with Paracandidimonas caeni 24 T (98.0%), followed by Pusillimonas soli MJ07T (97.5%), Parapusillimonas granuli Ch07T (97.2%), Pusillimonas ginsengisoli DCY25T (97.1%) and Paracandidimonas soli IMT-305 T (96.4%). The ANI values between strain Q2-2 T and the above related type strains were 71.02%, 73.52%, 74.32%, 74.59% and 72.29%, respectively. The DNA G + C content of strain Q2-2 T was 61.1%. Therefore, strain Q2-2 T represents a novel species of the genus Paracandidimonas, for which the name Paracandidimonas lactea sp. nov. (type strain Q2-2 T = CGMCC 1.19179 T = JCM 34906 T) is proposed.
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Abbreviations
- ANI:
-
Average nucleotide identity
- DPG:
-
Diphosphatidyl glycerol
- PE:
-
Phosphatidylethanolamine
- PME:
-
Phosphatidylmethylethanolamine
- PG:
-
Phosphatidyl glycerol
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Funding
This research is supported by The Key Research and Development Project of Zhejiang (Grant No. 2020C02030; 2021C02039) and Shanxi (Grant No. 2019TSLSF02-01-01) Provinces, The Open Project of Zhejiang Provincial Key Laboratory of Agricultural Green Biomanufacturing Core Strain Improvement (Grant No. 2020KFKT09), Zhejiang Provincial Natural Science Foundation of China (Grant No. LQ20C010006).
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X-fH and CS: designed the research and project outline. CZ, QP, J-jY, H-tW, YC and G-hZ: performed isolation, deposition and identifcation. CZ, C-zL, B-jW, Y-hZ and OL: performed fatty acids analysis. All authors read and approved the final manuscript.
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Zhang, C., Pu, Q., Ying, Jj. et al. Paracandidimonas lactea sp. nov., a urea-utilizing bacterium isolated from landfill. Arch Microbiol 204, 633 (2022). https://doi.org/10.1007/s00203-022-03190-4
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DOI: https://doi.org/10.1007/s00203-022-03190-4