Abstract
Paraburkholderia bengalensis sp. nov. strain IR64_4_BI was isolated from rice roots cultivated in Madhyamgram field station of Bose Institute, West Bengal, India. IR64_4_BI is a Gram-negative, motile, nitrate-reducing, nitrogen-fixing bacterium. Whole-cell fatty acid analyses of IR64_4_BI show C16:0, summed feature 8 (comprising C18:1ω7c and/or C18:1 ω 6c) and summed feature 3(C16:1 w7c/C16:1 w6c or C16:1 ω 7c/C16:1 ω 6c) were the predominant fatty acids. 16S rRNA phylogeny showed that it was most similar to P. phymatum STM815T (98.5% identity), P. terrae KMY02T (98.44% identity) and P. hospita LMG 20598T (98.32% identity). The Average Nucleotide Identity-BLAST (ANIb) of P. bengalensis IR64_4_BI with P. hospita DSM 17164T, P. terrae DSM 17804T, P. phymatum STM815T and P. hospita LMG 20598T was 83.11, 83.52, 84.5 and 83.12% respectively. Comparison of genome sequence of IR64_4_BI with other species of Paraburkholderia using the Multi-locus species tree software show that P. bengalensis IR64_4_BI is a novel species. The ability of P. bengalensis IR64_4_BI to survive on nitrogen-free medium under microaerophilic conditions and the abundance of nitrogen metabolism-related genes makes this strain a potential candidate for developing a nitrogen-fixing system in rice. Based on genotypic, phenotypic and chemotaxonomic studies, we propose that IR64_4_BI (= MTCC 13051 = JCM 34777) is a new species of Paraburkholderia which has been assigned as Paraburkholderia bengalensis sp.nov.
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Data availability
Repositories: 16S rRNA: MT912698. The whole Genome Shotgun project is available in GenBank under BioProject PRJNA647686. Strain deposited: MTCC, India: MTCC 13051; JCM, Japan: JCM 34777.
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Acknowledgements
The authors are very grateful for the help provided by Dr. Wriddhiman Ghosh, Department of Microbiology, Bose Institute for helping with genome sequencing of the strain. The authors acknowledges Mr. Swarnabha Das, Mr. Mrinal Das, Mr. Souvik Roy, Ms. Sheolee Ghosh Chakraborty,and Mr. Swaroop Biswas for their help. The authors would also like to thank Dr. Akansha Jain and Dr. Lekha Bandopadhyay for generously sharing chemicals. The authors are thankful to Prof. Aharon Oren, The Hebrew University of Jerusalem, for helping with the etymology. NM received fellowships from Science and Engineering Board, GoI and Bose Institute. JS obtained fellowships from Council of Scientific and Industrial Research, GoI. SD acknowledges Indian National Science Academy for providing INSA Senior Scientist Fellowship.
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PN acknowledges the DST-WOSA grant by Department of Science and Technology (SR/WOS-A/LS-463/2017).
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PN isolated the strain, designed and conducted the study and wrote the manuscript. NM and JS sequenced and assembled the genome, SD was involved in the critical assessment of the manuscript.
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Nag, P., Mondal, N., Sarkar, J. et al. Paraburkholderia bengalensis sp. nov. isolated from roots of Oryza sativa, IR64. Arch Microbiol 204, 347 (2022). https://doi.org/10.1007/s00203-022-02960-4
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DOI: https://doi.org/10.1007/s00203-022-02960-4